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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS196 |
| Replaces | 04-385 |
| Description | Anti-mTOR/FRAP Antibody, clone 22C2 |
| Alternate Names |
|
| Background Information | The mammalian target of rapamycin (mTOR), also known as FRAP, RAFT, or RAPT, is a ubiquitously expressed serine/threonine kinase that belongs to the phosphoinositide 3-kinase–related kinase (PIKK) family. mTOR functions as a downstream effector in the PI3K pathway and is activated by the Akt kinase. mTOR responds to signals from growth factors and promotes protein synthesis underlying cell proliferation, by activation of the S6 kinase and inhibition of the 4E-BP protein involved in protein translation. Several studies have suggested that the proliferative functions of mTOR may contribute to the development of cancer. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect the mTOR/FRAP protein using this Anti-mTOR Antibody, clone 22C2, validated for use in WB & IP. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: A representative lot was used by an independent laboratory in neuroblastoma cells. (Hosoi, H., et al. (1998). Molecular Pharmacology. 54:815–824.) Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in Rho30 cell extracts. (Hosoi, H., et al. (1998). Molecular Pharmacology. 54:815–824.) |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to human mTOR/FRAP. |
| Clone | 22C2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human. Predicted to react with Mouse and Rat based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| Molecular Weight | ~290 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in Jurkat cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected mTOR/FRAP on 10 µg of Jurkat cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |