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Millipore Detect Myod Using This Anti-Myod Antibody, Clone 5f11 Validated For Use In Western Blotting, Icc. - Mill (Additional S&H or Hazmat Fees May Apply)
Myoblast determination protein 1 (MyoD1) is a myogenic transcription factor required for the growth, repair, and regeneration of muscle cells. It induces differentiation of myoblast cells into multinucleated myotubules, activating genes and proteins involved in cell cycle arrest, and differentiation. MyoD1 may also control proliferation and homeostasis during embryogenesis, in the absence of Myf5.
Product Information
Format
Purified
Control
C2C12 cell lysate
Presentation
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect MyoD using this Anti-MyoD Antibody, clone 5F11 validated for use in Western Blotting, ICC.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A representative lot detected MyoD in C2C12 cells.
Western Blot Analysis: A representative lot detected MyoD in C2C12 cells.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to mouse MyoD.
Clone
5F11
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rat
Isotype
IgG2aκ
Species Reactivity
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_034996
Gene Symbol
Myod1
Myod
Purification Method
Protein G Purified
UniProt Number
P10085
Molecular Weight
~45 kDa observed. An uncharacterized band at ~38 kDa may be observed in some cell lysates. The calculated molecular weight is 34 kDa; however, this protein is known to be glycosylated.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in C2C12 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected MyoD in 10 µg of C2C12 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
