| Description | |
|---|---|
| Catalogue Number | S7701 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | TRAPeze® Telomerase Positive Control Cell Pellet |
| Overview | This product is intended for use with the components of the TRAPEZE® Telomerase Detection Kit (Catalog # S7700), the TRAPEZE® XL Telomerase Detection Kit (Catalog # S7707) and the TRAPEZE® ELISA Telomerase Detection Kit (Catalog # S7750). Recommended Taq polymerases: must be non-proofreading, having no exonuclease activity and capable of "hot-start." Titanium Taq™, Platinum® Taq are suggested. Material Provided Each vial of S7701 TRAPEZE® Positive Control Cell Pellet contains one million cells, pelletted to the bottom of the microcentrifuge tube. |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This product is intended for use with the components of the TRAPEZE Telomerase Detection Kit, the TRAPEZE XL Telomerase Detection Kit & the TRAPEZE ELISA Telomerase Detection Kit. |
| Application Notes | Instructions for Use 1. Resuspend the cell pellet in 200 μl of 1X CHAPS Lysis Buffer. 2. Incubate the suspension on ice for 30 minutes. 3. Spin the sample in a microcentrifuge at 12,000 X g for 20 minutes at 4°C. 4. Transfer 160 μl of the supernatant into a fresh tube. 5. Aliquot and quick-freeze the remaining extract on dry ice, and store at -75°C to -85°C. The extracts for the TRAP assay should be quick-frozen on dry ice after each use. Aliquots should not be freeze-thawed more than 5 times to avoid loss of telomerase activity. Additionally, aliquoting reduces the risk of contamination. 6. Use 1000 cell equivalents (2 μl of a 1:10 dilution) per TRAP assay, according to the kit manuals. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Must be stored at -75°C to -85°C. Telomerase in specimens stored as a cell pellet is stable at this temperature for several years. Once the cell extract is made (in 1X CHAPS Lysis Buffer), it is stable for 1 year, if not subjected to more than 5 freeze-thaw cycles |
| Packaging Information | |
|---|---|
| Material Size | 1 vial |
| Material Package | 1x10(e6) cells |