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Millipore Anti-Inntag™ 10 (It10), Clone It10 Antibody, Cat. No. Mac140-25ug, Validated For Use In Western Blotting And Immunocytochemistry. - Mill (Additional S&H or Hazmat Fees May Apply)
Pollen allergen Amb t 5 (UniProt: P10414; also known as Allergen Amb t V, Allergen Ra5G, Amb t 5) binds to IgE and causes allergic reaction in human. Inntag ™ IT10 is a small protein domain of Ambrosia trifida (Giant ragweed) that displays strong structural determinants and does not generate solubility issues in vivo and in vitro. Also, it does not compromise cell fitness when overexpressed, and has no detectable functional and localization effects when fused to a variety of target proteins. Structure of the Inntag ™ IT10 is based on a packed beta-sheet conformation.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Quality Level
MQ100
Applications
Application
Anti-Inntag™ 10 (IT10), clone IT10 Antibody, Cat. No. MAC140-25UG, validated for use in Western Blotting and Immunocytochemistry.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Western Blotting Analysis: A representative lot detected Inntag ™ 10 (IT10) in Western Blotting applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8). Immunocytochemistry Analysis: A representative lot detected Inntag ™ 10 (IT10) in Immunocytochemistry applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).
Biological Information
Immunogen
His-tagged recombinant peptide corresponding to 40 amino acids from Ambrosia trifida.
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone IT10-1 detects pollen allergen Abt t 5 in HEK293 cells transfected with GFP-IT10.
Isotype
IgG1
Species Reactivity
All
Species Reactivity Note
Ambrosia trifida.
Antibody Type
Monoclonal Antibody
Gene Symbol
N/A
Purification Method
Protein G purified
UniProt Number
P10414
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in transfected HEK293/GFP-IT10 cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
