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Millipore Anti-Inntag™ 6 (It6), Clone R19/4-11/15 Antibody, Cat. No. Mac139-25ug, Validated For Use In Western Blotting, Immunocytochemistry And Immunoprecipitation. - Mill (Additional S&H or Hazmat Fees May Apply)
Pro-hevein (UniProt: P02877; also known as Major hevein) is encoded by the HEV1 gene in Hevea brasiliensis (Para rubber tree). Pro-hevein is a N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin that can inhibit fungal growth. It is known to cause allergic reaction in human. It contains one barwin domain and one chitin-binding type-1 domain. Inntag ™ 6 IT6 is a small protein domain of Hevea brasiliensis that displays strong structural determinants and does not generate solubility issues in vivo and in vitro. Also, it is not shown to compromise cell fitness when overexpressed and has no detectable functional and localization effects when fused to a variety of target proteins. Unlike the monomeric HA, Flag and MYC tags, Inntag ™ IT6 does not increase the intrinsic variability of critical size in budding yeast cells. Structure of the Inntag ™ IT6 is based on a packed beta-sheet conformation.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG2b in PBS without azide.
Quality Level
MQ100
Applications
Application
Anti-Inntag™ 6 (IT6), clone R19/4-11/15 Antibody, Cat. No. MAC139-25UG, validated for use in Western Blotting, Immunocytochemistry and Immunoprecipitation.
Key Applications
Western Blotting
Immunocytochemistry
Immunoprecipitation
Application Notes
Western Blotting Analysis: A representative lot detected Inntag ™ 6 (IT6) in Western Blotting applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8). Immunoprecipitation Analysis: A representative lot detected Inntag ™ 6 (IT6) in Immunoprecipitation applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8). Immunofluorescence Analysis: A representative lot detected Inntag ™ 6 (IT6) in Immunofluorescence applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).
Biological Information
Immunogen
His-tagged recombinant peptide fragment corresponding to 43 amino acids from Hevea brasiliensis.
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone R19/4-11/15 detecs Pro-hevein in HEK293 cells tranfected with GFP-IT6.
Isotype
IgG2b
Species Reactivity
All
Species Reactivity Note
Hevea brasiliensis.
Antibody Type
Monoclonal Antibody
Gene Symbol
HEV1
Purification Method
Protein G purified
UniProt Number
P02877
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in transfected lysates from HEK293cells transfected with GFP-IT6.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
