Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | WB, ICC, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAC139 |
| Description | Anti-Inntag™ 6 (IT6) Antibody, clone R19/4-11/15 |
| Alternate Names |
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| Background Information | Pro-hevein (UniProt: P02877; also known as Major hevein) is encoded by the HEV1 gene in Hevea brasiliensis (Para rubber tree). Pro-hevein is a N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin that can inhibit fungal growth. It is known to cause allergic reaction in human. It contains one barwin domain and one chitin-binding type-1 domain. Inntag 6 IT6 is a small protein domain of Hevea brasiliensis that displays strong structural determinants and does not generate solubility issues in vivo and in vitro. Also, it is not shown to compromise cell fitness when overexpressed and has no detectable functional and localization effects when fused to a variety of target proteins. Unlike the monomeric HA, Flag and MYC tags, Inntag IT6 does not increase the intrinsic variability of critical size in budding yeast cells. Structure of the Inntag IT6 is based on a packed beta-sheet conformation. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2b in PBS without azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Inntag 6 (IT6), clone R19/4-11/15 is a mouse monoclonal antibody that detects the Innocuous Inntag IT6 wherein the tag protein is a fragment of Hev b 6.02 of Hevea brasiliensis. It has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected Inntag 6 (IT6) in Western Blotting applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8). Immunoprecipitation Analysis: A representative lot detected Inntag 6 (IT6) in Immunoprecipitation applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8). Immunofluorescence Analysis: A representative lot detected Inntag 6 (IT6) in Immunofluorescence applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged recombinant peptide fragment corresponding to 43 amino acids from Hevea brasiliensis. |
| Clone | R19/4-11/15 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone R19/4-11/15 detecs Pro-hevein in HEK293 cells tranfected with GFP-IT6. |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Hevea brasiliensis. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
|
| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~34.1 kDa observed; 21.86 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in transfected lysates from HEK293cells transfected with GFP-IT6. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Inntag 6 (IT6) in 10 µg lysate from HEK293 cells transfected with GFP-IT6. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |