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Millipore Anti-Inntag™ 5 (It5), Clone R19/8-11/18 Antibody, Cat. No. Mac138-25ul, Validated For Use In Western Blotting, Immunofluorescence And Immunoprecipitation. - Mill (Additional S&H or Hazmat Fees May Apply)
Pollen allergen Phl p 2 (UniProt: P43214; also known as Allergen Phl p II, Phl p 2) is encoded by the PHLPII gene in Phleum pratense (Common timothy). Phl p2 binds to IgE and causes allergic reactions in human. Inntag ™ 5 IT5 is a small protein domain of Phelum pratense that display strong structural determinants and does not generate solubility issues in vivo and in vitro. Also, it is not shown to compromise cell fitness when overexpressed and has no detectable functional and localization effects when fused to a variety of target proteins. Although the Inntag ™ IT5 has a similar length to that of the Flag tag, it does not affect cell morphology and produces only very mild effect in the mean critical size at the G1/S transition compared to Flag tag. The structure of Inntag ™ IT5 is based on a packed beta-sheet confirmation.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG2b in PBS without azide.
Quality Level
MQ100
Applications
Application
Anti-Inntag™ 5 (IT5), clone R19/8-11/18 Antibody, Cat. No. MAC138-25UL, validated for use in Western Blotting, Immunofluorescence and Immunoprecipitation.
Key Applications
Western Blotting
Immunofluorescence
Immunoprecipitation
Application Notes
Western Blotting Analysis: A representative lot detected Inntag ™ 5 (IT5) in Western Blotting applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8). Immunoprecipitation Analysis: A representative lot detected Inntag ™ 5 (IT5) in Immunoprecipitation applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8). Immunofluorescence Analysis: A representative lot detected Inntag ™ 5 (IT5) in Immunofluorescence applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).
Biological Information
Immunogen
His-tagged recombinant fragment corresponding to 96 amino acids of Pollen allergen Phl p 2 chain.
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone R19/8-11/18 detects Pollen allergen Phl p 2 in lysate from HEK293 cells transfected with GFP-IT5.
Isotype
IgG2b
Species Reactivity
All
Species Reactivity Note
Phaleum pratense.
Antibody Type
Monoclonal Antibody
Gene Symbol
PHLPII
Purification Method
Protein G purified
UniProt Number
P43214
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in transfected HEK293/GFP-IT5 cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
