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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | WB, ICC, IP, ELISA | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAC111 |
| Description | Anti-GST•Tag™ fusion protein Antibody |
| Alternate Names |
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| Background Information | GST (glutathione-S-transferase) represents a major group of detoxification enzymes, which are known to exist as multiple cytosolic and membrane-bound isoenzymes, with distinct catalytic and noncatalytic binding properties. Whereas the cytosolic enzymes are encoded by at least five distantly related gene families, the membrane-bound enzymes are encoded by single genes. GST is regulated and induced by a diverse range of xenobiotics. Many, but not all, inducers of GST, act through either the antioxidant-responsive element (ARE), the GST P enhancer 1(GPE), the xenobiotic-responsive element (XRE), or the glucocorticoid-responsive element (GRE). Barbiturates may transcriptionally activate GST through a Barbie box element. Many of the compounds that induce GST are also GST substrates, or are metabolized by cytochrome P-450 monooxygenases into compounds that can serve as GST substrates. Because the expression levels of GST play a critical role in determining the sensitivity of cells to a broad range of toxic chemicals, GST is suggested to contribute to resistance against carcinogens, antitumor drugs, environmental pollutants, and products of oxidative stress. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ cultured supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-GST•Tag fusion protein Antibody is validated for use in Western Blotting, ICC, Immunoprecipitation, ELISA for the detection of GST•Tag fusion protein. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A representative lot detected GST•Tag fusion protein in transfected COS-1 cells. Immunoprecipitation Analysis: A representative lot immunoprecipitated GST•Tag fusion protein sequence in IP. ELISA Analysis: A representative lot detected GST•Tag protein sequence in ELISA. |
| Biological Information | |
|---|---|
| Immunogen | Gluththione-S-transferase (GST) domain from Schistosoma Japonicum |
| Clone | 8-326.6.1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~45 kDa observed. The molecular weight of the GST•Tag fusion protein is not ~45 kDa. The Posi-Tag Epitope Tag Control, which contains the fusion protein, is observed at ~45 kDa in Western Blot. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in Posi-Tag Epitope Tag Control. Western Blot Analysis: This antibody detected GST•Tag fusion protein in 5 µL of Posi-Tag Epitope Tag Control. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |