Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | IH(P), IF, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT917 |
| Description | Anti-Connexin 43 Antibody, clone 2F5 |
| Alternate Names |
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| Background Information | Gap junction alpha-1 protein (UniProt: P08050; also known as Connexin-43, CX43, Gap junction 43 kDa heart protein) is encoded by the Gja1 (also known as Cxn-43) gene (Gene ID: 24392) in rat. The gap junction channels are dodecamers of transmembrane proteins of the connexin family. Connexins usually have a short half-life ( 1.5 - 5 h) and this short half-life is a key element of coupling regulation as it allows very dynamic and acute changes in gap junction regulation and gap junctional communication. In cardiomyocytes three types of connexins have been identified: Connexin 40, 43, and 45. Each of these connexins form channels with unique and specific electrophysiological properties. Connexin-43 is a multi-pass membrane protein and is the most ubiquitous connexin with widespread tissue expression pattern. Connexin-43 can be phosphorylated at multiple sites and phosphorylation is shown to regulate the kinetics of its trafficking, assembly, gating and turnover in a cell-cycle-stage specific manner. Phosphorylation at Ser325, Ser328 and Ser330 by casein kinase I is reported to modulate gap junction assembly while phosphorylation of Ser368 by protein kinase C gamma isoform leads to disassembly of gap junction plaques and inhibition of gap junction activity. During myocardial ischemia connexin-43 is shown to become dephosphorylated that allows gap junctions to be opened and convey the propagation of ischemia injury. In bladder smooth muscle cells, connexin-43 displays a circadian cycle with low levels during the sleep phase. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This mouse monoclonal Anti-Connexin 43, clone 2F5, Cat. No. MABT917 is tested for use in Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting for the detection of Connexin-43. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Connexin 43 in rat heart tissue. Western Blotting Analysis: A representative lot detected Connexin 43 in mouse heart tissue lysates, NRK cell lysates, and MDCK cell lysates (Courtesy of Joell Solan in Paul Lampes Lab at Fred Hutchinson Cancer Research Center, Seattle, WA). Immunofluorescence Analysis: A representative lot detected Connexin 43 in NRK cells (Courtesy of Joell Solan in Paul Lampes Lab at Fred Hutchinson Cancer Research Center, Seattle, WA). |
| Biological Information | |
|---|---|
| Immunogen | A recombinant fragment corresponding to 136 amino acids from the C-terminal half of Rat connexin-43. |
| Epitope | C-terminus |
| Clone | 2F5 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 2F5 detects connexin-43 in human and rat heart. It targets an epitope within 136 amino acids from the C-terminal half. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Rat |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 43.03 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human heart tissue. Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Connexin 43 in human heart tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |