Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, FC, WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT914 |
| Description | Anti-CD9 Antibody, clone Z9.1 |
| Alternate Names |
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| Background Information | CD9 antigen (UniProt: P21926; also known as 5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; MRP-1; Tetraspanin-29; Tspan-29; p24) is encoded by the CD9 (also known as MIC3, TSPAN29; IG2) gene (Gene ID: 928) in human. CD9 is a multi-pass membrane glycoprotein of the tetraspanin (TM4SF) family. It is expressed in a variety of hematopoietic and epithelial cells. It displays strong expression in platelets where it mediates platelet activation and aggregation upon binding to monoclonal antibodies. CD9 induces cell-cell adhesion, platelet aggregation, and B cell development and is shown to associate with CD63, CD81, CD82, and CD36 and to bind to beta-1 integrins. CD9 activation of platelets is reported to be mediated by the Fc y RII (CD32) molecule. Downregulation of CD9 correlates with tumor progression in some forms of cancer. It can hamper metastasis formation by inhibiting integrin-mediated motility. Lower expression of CD9 are associated with weaker matrix adhesion and diffuse tumor cell growth in vitro. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This mouse monoclonal Anti-CD9 antibody, clone Z9.1, Cat. No. MABT914, is validated for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting for the detection of CD9. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected CD9 in human tonsil, human breast cancer, and human skin tissues. Flow Cytometry Analysis: A 1:1,000 dilution from a representative lot detected CD9 in human PBMCs. human tonsil, human breast cancer, human liver cancer, human skin |
| Biological Information | |
|---|---|
| Immunogen | Intact LoVo colorectal carcinoma cells. |
| Epitope | extracellular domain |
| Clone | Z9.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone Z9.1 detects CD9 in its native, non-reduced form, in CD-9 positive cells amongst human PBMCs. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 25.42 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human human liver cancer tissue. Immunohistochemistry Analysis: A 1:1000 dilution of this antibody detected CD9 in human liver cancer tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 50 μg |