Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H, R | WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT903 |
| Description | Anti-Connexin 43 Antibody, N-terminal Antibody, clone P1E11.B19 |
| Alternate Names |
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| Background Information | Gap junction alpha-1 protein (UniProt: P08050; also known as Connexin-43, CX43, Gap junction 43 kDa heart protein) is encoded by the Gja1 (also known as Cxn-43) gene (Gene ID: 24392) in rat. The gap junction channels are dodecamers of transmembrane proteins of the connexin family. Connexins usually have a short half-life ( 1.5 - 5 h) and this short half-life is a key element of coupling regulation as it allows very dynamic and acute changes in gap junction regulation and gap junctional communication. In cardiomyocytes three types of connexins have been identified: Connexin 40, 43, and 45. Each of these connexins form channels with unique and specific electrophysiological properties. Connexin-43 is a multi-pass membrane protein and is the most ubiquitous connexin with widespread tissue expression pattern. Connexin-43 can be phosphorylated at multiple sites and phosphorylation is shown to regulate the kinetics of its trafficking, assembly, gating and turnover in a cell-cycle-stage specific manner. Phosphorylation at Ser325, Ser328 and Ser330 by casein kinase I is reported to modulate gap junction assembly while phosphorylation of Ser368 by protein kinase C gamma isoform leads to disassembly of gap junction plaques and inhibition of gap junction activity. During myocardial ischemia connexin-43 is shown to become dephosphorylated that allows gap junctions to be opened and convey the propagation of ischemia injury. In bladder smooth muscle cells, connexin-43 displays a circadian cycle with low levels during the sleep phase. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This mouse monoclonal Anti-Connexin 43, N-terminal, clone P1E11.B19, Cat. No. MABT903 is tested for use in Immunocytochemistry and Western Blotting for the detection of Connexin-43. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected Connexin 43 in Western Blotting applications (Sosinsky, G.E., et. al. (2007). Biochem. 408(3):375-85). Western Blotting Analysis (WB): A representative lot detected Connexin 43 in NRK cells (Courtesy of Joell Solan in Paul Lampe s Lab at Fred Hutchinson Cancer Research Center, Seattle, WA). Western Blotting Analysis: A representative lot detected Connexin 43 in Western Blotting applications (Kalcheva, N., et. al. (2007). Proc Natl Acad Sci USA. 104(51):20512-6). Western Blotting Analysis: 1-4 µg/mL from a representative lot detected Connexin 43 in 10 µg of rat and mouse heart tissue lysates. Immunocytochemistry Analysis: A representative lot detected Connexin 43 in Immunocytochemistry applications (Gellhaus, A., et. al. (2004). J Biol Chem. 279(35):36931-42). Western Blotting Analysis: A representative lot detected Connexin 43 in Western Blotting applications (Goldberg, G.S., et. al. (2002). J Biol Chem. 277(39):36725-30). Western Blotting Analysis: A representative lot detected Connexin 43 in Western Blotting applications (Solan, J.L., et. al. (2003). J Cell Sci. 116(Pt 11):2203-11). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the first 20 amino acids from the N-terminal region of rat connection-43. |
| Epitope | N-terminus |
| Clone | P1E11 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone P1E11.B19 detects connexin-43 in most mammalian species. It targets an epitope with in the first 20 amino acids from the N-terminal end. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse, Rat. Predicted to react with Bovine, Canine, Chicken, Hamster, Horse, Porcine, Rabbit, and Rhesus Macaque based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~43 kDa observed; 43.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human heart tissue lysates. Western Blotting Analysis: 1 µg/mL of this antibody detected Connexin 43 in 10 µg of human heart tissue lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |