Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | ICC, IP, IH(P), IF | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT901 |
| Description | Anti-Connexin 43 Antibody, C-terminal Antibody, clone P4G9 |
| Alternate Names |
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| Background Information | Gap junction alpha-1 protein (UniProt: P08050; also known as Connexin-43, CX43, Gap junction 43 kDa heart protein) is encoded by the Gja1 (also known as Cxn-43) gene (Gene ID: 24392) in rat. The gap junction channels are dodecamers of transmembrane proteins of the connexin family. Connexins usually have a short half-life ( 1.5 - 5 h) and this short half-life is a key element of coupling regulation as it allows very dynamic and acute changes in gap junction regulation and gap junctional communication. In cardiomyocytes three types of connexins have been identified: Connexin 40, 43, and 45. Each of these connexins form channels with unique and specific electrophysiological properties. Connexin-43 is a multi-pass membrane protein and is the most ubiquitous connexin with widespread tissue expression pattern. Connexin-43 can be phosphorylated at multiple sites and phosphorylation is shown to regulate the kinetics of its trafficking, assembly, gating and turnover in a cell-cycle-stage specific manner. Phosphorylation at Ser325, Ser328 and Ser330 by casein kinase I is reported to modulate gap junction assembly while phosphorylation of Ser368 by protein kinase C gamma isoform leads to disassembly of gap junction plaques and inhibition of gap junction activity. During myocardial ischemia connexin-43 is shown to become dephosphorylated that allows gap junctions to be opened and convey the propagation of ischemia injury. In bladder smooth muscle cells, connexin-43 displays a circadian cycle with low levels during the sleep phase. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This mouse monoclonal Anti-Connexin 43, C-terminal, clone P4G9, Cat. No. MABT901 is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Immunoprecipitation for the detection of Connexin-43. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Connexin 43 in rat heart tissue. Immunofluorescence Analysis: A representative lot detected Connexin 43 in NRK cells (Courtesy of Joell Solan in Paul Lampe s Lab at the Fred Hutchinson Cancer Research Center, Seattle, WA). Immunoprecipitation Analysis: A representative lot detected Connexin 43 in Immunoprecipitation applications (Sosinsky, G.E., et. al. (2007). Biochem J. 408(3):375-85). Immunocytochemistry Analysis: A representative lot detected Connexin 43 in Immunocytochemistry applications (Norris, R.P., et. al. (2008). Development. 135(19):3229-38). Immunocytochemistry Analysis: A representative lot detected Connexin 43 in Immunocytochemistry applications (Churko, J.M., et. al. (2010). Biochem J. 429(3):473-83). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal region of rat connexin-43. |
| Epitope | C-terminus |
| Clone | P4G9 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone P4G9 specifically detects connexin 43 in human and rat heart. It targets an epitopw within 23 amino acids from the C-terminal end. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Rat, Mouse. Predicted to react with Bovine, Canine, Guinea Pig, Hamster, Porcine, Rabbit, and Rhesus Moneky based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 43.03 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human heart tissue. Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Connexin 43 in human heart tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |