Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT900 |
| Description | Anti-Connexin 45 Antibody, clone P3C9 |
| Alternate Names |
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| Background Information | Gap junction gamma-1 protein (UniProt: A4GG66; also known as Connexin-45, Cx45, Gap junction alpha-7 protein) is encoded by the Gjc1 (also known as Gja7) gene (Gene ID: 266706) in rat. The gap junction channels are dodecamers of transmembrane proteins of the connexin family. Connexins usually have a short half-life ( 1.5 - 5 h) and this short half-life is a key element of coupling regulation as it allows very dynamic and acute changes in gap junction regulation and gap junctional communication. In cardiomyocytes three types of connexins have been identified: Connexin 40, 43, and 45. Each of these connexins form channels with unique and specific electrophysiological properties. Connexin-45 is a multi-pass membrane protein with ubiquitous expression. It consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low molecular weights diffuse from one cell to a neighboring cell. Connexin 45 is the first cardiac connexin expressed during embryonic development and plays an important role in propagating the action potential from the conduction system to the working myocardium. Connexin 45 gap junction channels are reported to close when the membrane potential becomes negative. This prevents retrograde conduction from myocardium to the conduction system. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This mouse monoclonal Anti-Connexin 45, clone P3C9, Cat. No. MABT900 is tested for use in Western Blotting for the detection of Connexin-45. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected Connexin-45 in Western Blotting applications (Manias, J.L., et. al. (2008). Cardiovasc Res. 80(3):385-95). Western Blotting Analysis: A representative lot detected Connexin-45 in Western Blotting applications (Kopanic, J.L., et. al. (2013). Front Pharmacol. 4:106). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal half of rat connexin 45. |
| Clone | P3C9 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone clone P3C9 detects Connexin-45 in rat and mouse cells. It targets an epitope within 14 amino acids from the C-terminal half of rat connexin 45. |
| Isotype | IgG2bκ |
| Species Reactivity |
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| Species Reactivity Note | Rat, Mouse. Predicted to react with Bovine, Canine, Chicken, Human, Porcine, Rabbit, and Rhesus Macaque based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~45 kDa observed; 45.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse heart tissue lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected Connexin 45 in 10 µg of mouse heart tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |