Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT899 |
| Description | Anti-Mucin 5B Antibody, clone MDA-3E1 |
| Alternate Names |
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| Background Information | Mucin 5, subtype B, tracheobronchial (UniProt: E9Q5I3; also known as MUC5B ) is encoded by the Muc5b gene (Gene ID: 74180) in murine species. Mucins are high molecular weight glycoproteins that consist of a mucin core protein and O-linked carbohydrates. Mucins are present on the surface of, and are secreted by, many glandular epithelial cells. They provide a barrier between the luminal membranes of cells in the epithelium and their environment. Mucins consist of a nonglobular protein backbone (apomucin) and many O-linked glycans. The two principal polymeric mucins are reported in the airways. They are Mucin 5ac that is produced at low levels under healthy conditions, but increases during allergic inflammation or parasitic infection, and Mucin 5B that is produced at substantial levels under healthy conditions and modestly increases under conditions of allergic inflammation Mucin 5B is shown to be abnormally expressed in gastric carcinoma. (Ref.: Ren, B et al. (2015). (Biosci Rep. 35(3): e00220). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Mucin 5B, clone MDA-3E1 , Cat. No. MABT899, is a mouse monoclonal antibdoy that detects Mucin 5b and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A representative lot detected Muc5b in Immunohistochemistry applications (Ren, B., et. al. (2015). Biosci Rep. 35(3), e00220). Western Blotting Analysis: A representative lot detected Muc5b in Western Blotting applications (Ren, B., et. al. (2015). Biosci Rep. 35(3); e00220). |
| Biological Information | |
|---|---|
| Immunogen | A linear peptide corresponding to 20 amino acids from the N-terminal half of murine Mucin 5 subunit B. |
| Clone | MDA-3E1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone MDA-3E1 detects Mucin 5b in human and murine species, it targets an epitope within 20 amino acids from the N-terminal half. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 515.44 kDa calculated. The actual molecular weight of the glycosylated protein may be up to 10-fold higher. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human nasal mucosal tissue. Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Muc5b in human nasal mucosal tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 200 µL |