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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M | ICC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT892 |
| Description | Anti-SUN1 Antibody, clone 12.10F |
| Alternate Names |
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| Background Information | SUN domain-containing protein 1 (UniProt Q9D666; also known as Protein unc-84 homolog A, Sad1/unc-84 protein-like 1) is encoded by the Sun1 (also known as Unc84a) gene (Gene ID 77053) in murine species. SUN1 is a component of the SUN-protein-containing complexes known as LINC (linker of nucleoskeleton and cytoskeleton), which link the nucleoskeleton and cytoskeleton structures together in a scaffold like fashion. SUN1 is required for interkinetic nuclear migration and nucleokinesis as well as nucleus-centrosome coupling during radial neuronal migration and glial migration in the cortex. SUN1 also is important during meiosis and is required for proper gametogenesis. It also participates in anchoring chromosome movement in the prophase of meiosis and in selective gene expression of coding and non-coding RNAs required for gametogenesis. Additionally, it has a vital role in the distribution of nuclear pore complexes on the nuclear membrane. Interestingly, LINC complexes are also critical for vital functions such as hearing. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mous IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This mouse monoclonal Anti-SUN1 Antibody, clone 12.10F, Cat. No. MABT892 is validated for use in Immunocytochemistry and Western Blotting for the detection of SUN1. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected SUN1 nuclear immunoreactivity in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized C2C12 murine myoblasts. Immunocytochemistry Analysis: A representative lot immunostained nucleus of C2C12 murine myoblasts (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR, Singapore). Western Blotting Analysis: A representative lot detected greatly reduced SUN1 isoforms target bands in lysate from SUN1 siRNA-treated C2C12 murine myoblasts (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR, Singapore). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant fusion protein composed of murine SUN1 regions encoded by exons 6, 8, and 10. |
| Clone | 12.10F |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 12.10F was raised against a fusion construct composed of murine SUN1 exon 6-, 8-, and 10-encoded regions that are present in murine spliced isoforms 1, 3, and 4 reported by UniProt (Q9D666). The epitope location among these three regions has not been mapped and reactivity toward murine spliced isoform 3 is expected only if the epitope resides within exon 1- or 4-encoded region. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Mouse, Rat. Not Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~102 kDa oberved. 102.0/88.20/97.92/94.51 kDa (mouse isoform 1/2/3/4) calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in C2C12 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected SUN1 in 10 µg of C2C12 murine myoblast lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |