Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Op, R, M | IF, WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT840 |
| Description | Anti-Myosin-2 (MYH2) Antibody, clone SC-71 |
| Alternate Names |
|
| Background Information | Myosin-2 (UniProt Q9BE41; also known as MyHC-2a, MyHC-IIa, Myosin heavy chain 2, Myosin heavy chain 2a, Myosin heavy chain IIa, Myosin heavy chain, skeletal muscle, adult 2) is encoded by the MYHSA gene (Gene ID 788772) in bovine species. Myosin heavy chain (MyHC) is a major structural component of the striated muscle contractile apparatus and is essential for body movement and cardiac contractility. MyHC are encoded by a highly conserved multigene family, of which eight isoforms have been identified in mammals, each encoded by a separate gene that displays distinct temporal-spatial regulation. MyHC-2a is expressed in the fast-type 2A muscle fibers. Recessive MyHC-2a myopathy caused by missense mutations results in mild muscle weakness. Autosomal dominant mutation (E706K) is reported to cause defective muscle function and compromises the structural integrity of all muscle cells. Ref.: Tajsharghi, H et al. (2014). Eur. J. Hum. Genet. 22: 801-808. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This mouse monoclonal Anti-Myosin-2 (MYH2) Antibody, clone SC-71, Cat. No. MABT840, is validated for use in Immunofluorescence, Immunohistochemistry, and Western Blotting for the detection of Myosin-2. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Myosin-2 (MYH2) in 10 µg of human gastrocnemious muscle and showed minimal reactivity with soleus embryonic muscle tissue lysates (Courtesy of Alberto Rossi, Ph.D., University of Colorado, U.S.A.). Immunofluorescence Analysis: A representative lot immunostained type 2A fibers in mouse hindlimb muscle cryosections encompassing soleus and surrounding tissues (Kurapati, R., et al. (2012). Hum. Mol. Genet. 21(8):1706-1724). Immunofluorescence Analysis: A representative lot immunostained type 2A fibers in rat soleus muscle cryosections following Bupivacaine-induced muscle regeneration. In tetrodotoxin/TTX-paralyzed-regenerated muscles type 2A MHC was not expressed (Midrio, M., et al. (2002). Basic Appl. Myol. 12(2): 77-80). Immunohistochemistry Analysis: A representative lot detected type IIA myosin heavy chain (MyHC) in human masseter (jaw) muscle cryosections (Horton, M.J., et al. (2001). Arch. Oral Biol. 46(11):1039-1050). Immunohistochemistry Analysis: Representative lots immunostained type 2A, but not type 1, 2B, or 2X, fibers in soleus (rat) and tibialis (mouse, rat, and Mgray short-tailed opossum/Monodelphis domestica) anterior muscle cryosections. Clone SC-71 failed to stain guinea pig tibialis sections (Sciote, J.J., and Rowlerson, A. (1998). Anat. Rec. 251(4):548-562; Gorza, L. (1990). J. Histochem Cytochem. 38(2):257-265; Schiaffino, S., et al. (1989). J. Muscle Res. Cell Motil. 10(3):197-205). Western Blotting Analysis: A representative lot detected type IIA myosin heavy chain (MyHC) in human masseter (jaw) single muscle fibres extract (Horton, M.J., et al. (2001). Arch. Oral Biol. 46(11):1039-1050). Western Blotting Analysis: A representative lot detected myosin heavy chain (MHC) in myosin preparations from rat diaphragm, as well as the light meromyosin and rod, but not heavy meromyosin or S-1, fragments of MHC (Schiaffino, S., et al. (1989). J. Muscle Res. Cell Motil. 10(3):197-205). |
| Biological Information | |
|---|---|
| Immunogen | Bovine skeletal muscle myosin (Schiaffino, S., et al. (1989). J. Muscle Res. Cell Motil. 10(3):197-205). |
| Clone | SC-71 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone SC-71 immunostained type 2A, but not type 1, 2B, or 2X, fibers in rat tibialis anterior muscle by targeting an epitope within the light meromyosin region (Schiaffino, S., et al. (1989). J. Muscle Res. Cell Motil. 10(3):197-205). |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Opossum, Rat. Not Guinea pig. Predicted to react with Bovine based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G purified. |
| UniProt Number |
|
| Molecular Weight | ~225 kDa observed. 223.3 kDa (bovine), 223.0 (human), 223.2 kDa (mouse) calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Identity Confirmation by Isotyping Test. Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 . |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |