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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC, FC, ELISA | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT65 |
| Description | Anti-CEACAM1 Antibody, clone 4D1/C2 |
| Alternate Names |
|
| Background Information | CEACAM1 (carcinoembryonic antigen-related cell adhesion molecule 1) is thought to be a member of the immunoglobulin superfamily and is known as an epithelial tumor suppressor and an angiogenic growth factor. It has also been linked to the actin-based cytoskeleton. CEACAM1 is also known as a cellular receptor for a number of human mucosa pathogenic bacteria. The loss of activity of CEACAM1 has been related to the development of colorectal cancer. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect CEACAM1 using this Anti-CEACAM1 Antibody, clone 4D1/C2 validated for use in WB, IH. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A representative lot was used by an independent laboratory to detect CEACAM1 in non-small-cell lung cancer (Dango, S., et al. (2008). Lung Cancer. 60:426-433). Western Blot Analysis: A representative lot was used by an independent laboratory to detect CEACAM1 in human granulocyte lysate (Bogoevska, V. et al., (2006). Glycobiology. 16(3): 197–209). Flow Cytometry Analysis: A representative lot was used by an independent laboratory to detect CEACAM1 in human granulocytes by FACS analysis (Bogoevska, V. et al., (2006). Glycobiology. 16(3): 197–209). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human CEACAM1. |
| Epitope | Unknown |
| Clone | 4D1/C2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of all variants has not been defined. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein Isoform 2: Secreted Isoform 3: Secreted Isoform 4: Secreted Isoform 5: Cell membrane; Single-pass type I membrane protein Isoform 6: Cell membrane; Single-pass type I membrane protein Isoform 7: Cell membrane; Single-pass type I membrane protein Isoform 8: Cell membrane; Single-pass type I membrane protein. Note: Localizes to sites of cell-cell contact. SEQUENCE SIMILARITIES: Belongs to the immunoglobulin superfamily. CEA family. Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SEQUENCE CAUTION: The sequence AAA57141.1 differs from that shown. Reason: Erroneous gene model prediction. |
| Molecular Weight | ~150 kDa observed. Bands are usually observed at ~120-160 kDa, resulting from different glycosylations (Lauke, H., et al. (2004). Mol. Hum. Reprod. 10(4):247-252). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HepG2 cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected CEACAM1 in 10 µg of HepG2 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |