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Millipore This Mouse Monoclonal Anti-Lamin A/C Antibody, Clone 2e8.2, Cat. No. Mabt538 Is Validated For Use In Immunohistochemistry (Paraffin) And Western Blotting For The Detection Of Lamin A/C. - Mill (Additional S&H or Hazmat Fees May Apply)
Lamin-A is a key component of the nuclear lamina, a protein network underlying the inner membrane that determines nuclear shape and size. The same gene (Lmna, Dhe, or Lamn1 in murine species; Gene ID 16905) that encodes lamin-A also codes for the alternatively spliced prelamin-C isoforms (UniProt P48678-2 and P48678-3). Murine lamin-A (UniProt P48678-1) is initially produced as a 665-amino acid prelamin A, which is further processed posttranslationally by four enzymatic steps to yield the mature Lamin-A. Farnesylation of Cys662, endoproteolytic cleavage of the last three amino acids (a.a. 663-665), carboxyl methylation of Cys662, and the final endoproteolytic removal of the remaining propeptide sequence (a.a. 648-662), including the farnesylated Cys662, catalyzed by the ER membrane zinc metalloproteinase ZMPSTE24.
Product Information
Format
Purified
Presentation
Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This mouse monoclonal Anti-Lamin A/C Antibody, clone 2E8.2, Cat. No. MABT538 is validated for use in Immunohistochemistry (Paraffin) and Western Blotting for the detection of lamin A/C.
Key Applications
Immunohistochemistry (Paraffin)
Western Blotting
Application Notes
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot immunostained nuclear membrane in human cerebellum, kidney, and pancreas tissue sections.
Biological Information
Immunogen
GST-tagged recombinant fragment within the C-terminal half of human Lamin A.
Clone
2E8.2
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 2E8.2 immunostained nuclear membrane in human tissue sections.
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_733821
Gene Symbol
LMNA
LMN1
Purification Method
Protein G purified.
UniProt Number
P02545
Molecular Weight
~70/65 kDa observed. 74.14/73.81/72.22 kDa (perpro-/pre-/mature Lamin A) and 65.14 kDa (Lamin C) calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected lamin A/C in 10 µg of A431 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
