Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT535 |
| Description | Anti-Desmin Antibody, clone 4C12.1 |
| Background Information | Desmin (UniProt: P17661) is encoded by the DES gene (Gene ID: 1674) in human. Desmin is a major class III intermediate filament protein essential for the structural integrity and function of muscle. In adult striated muscle desmin forms a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. It may also act as a sarcomeric microtubule-anchoring protein and specifically associate with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Desmin is particularly abundant at the myotendinous junctions and at the neuromuscular junctions of skeletal muscle. Mammalian cardiac muscle cells contain higher amounts of desmin (up to 2% of total protein) compared to skeletal muscle cells (0.35% of total protein). Mutations in DES gene have been linked to familial cardiac and skeletal myopathy characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, and restrictive heart failure. It can also lead to myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells. (rEF.: Denise Paulin, D., and Li, Z (2004). Experimental Cell Research 301(1); 1 -7). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Desmin Antibody, clone 4C12.1, Cat. No. MABT535, is a mouse monoclonal antibody that targets Desmin in Human, Mouse, and Rat and it is tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Desmin in mouse heart, mouse skeletal muscle, rat heart, and rat skeletal muscle tissues. Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Desmin in human heart and human skeletal muscle tissues. |
| Biological Information | |
|---|---|
| Immunogen | GST/His-taged recombinant fragment corresponding to 64 amino acids from the C-terminal region of human Desmin. |
| Clone | 4C12.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 4C12.1 specifically detects Desmin in human, mouse, and rat muscles. It targets an epitope within 64 amino acids from the C-terminal end. |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~53 kDa observed; 53.53 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human skeletal muscle tissue lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Desmin in 10 µg of human skeletal muscle tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |