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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ELISA | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT418 |
| Replaces | MABT473 |
| Description | Anti-GPVI Antibody, clone 8A2, Detection Antibody |
| Alternate Names |
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| Background Information | Glycoprotein VI (GPVI), or glycoprotein 6 (GP6), is a member of the immunoglobulin gene superfamily, closely related to Fc receptor gamma chain (FcRgamma) and natural killer receptors. GPVI is a collagen receptor involved in collagen-induced platelet adhesion and activation, and may contribute to arterial and venous thrombus formation. GPVI is a membrane glycoprotein that plays a crucial role in platelet procoagulant activity and subsequent thrombin and fibrin formation. GPVI is expressed in megakaryocytes and platelets. The signaling pathway of GPVI involves the FcRgamma, the Src kinases (likely Fyn/Lyn), the adapter protein LAT and leads to the activation of phospholipase C gamma2. It has been shown that bleeding disorders such as Bleeding Disorder Platelet-Type 11 (BDPLT11), a mild to moderate bleeding disorder caused by defective platelet activation and aggregation in response to collagen, is caused by defects in GPVI. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-GPVI Antibody, clone 8A2, Detection Antibody is an antibody against GPVI for use in western blotting & ELISA. |
| Key Applications |
|
| Application Notes | ELISA Assay: A representative lot from an independent laboratory detected recombinant GPVI in a bead-based sandwich immunoassay (Bigalke, B., et al. (2011). Clin Chem. 57(6):898-904.). This antibody should be used in conjunction with Cat. No. MABT406, Anti-GPVI, clone 8E9, Capture Antibody, as an ELISA detection and capture antibody pair. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human GPVI. |
| Clone | 8A2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rat |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~50 kDa observed. The calculated molecular weight of this protein is 37 kDa. However, a recombinant protein was observed at 50-55 kDa in Western Blotting. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in recombinant human GPVI glycoprotein. Western Blotting Analysis: 0.5 µg/mL of this antibody detected recombinant human GPVI glycoprotein. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |