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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT390 |
| Description | Anti-Syntaxin-2 Antibody, clone 2F2.1 |
| Alternate Names |
|
| Background Information | The protein named Syntaxin-2 or Epimorphin is a multifunctional membrane protein with dual topology. Its C terminus is membrane anchored. When the N terminus is on the cytosolic face it acts as a t-SNARE involved in intracellular vesicle docking and is called Syntaxin-2. When flipped inside out the N terminus hangs out on the extracellular surface (by some nonclassical secretion pathway) it acts as a versatile morphogen and is called Epimorphin. This membrane protein enjoys the double choice of another form of topological alternatives of being targeted to either apical or basolateral surface of an epithelial cell in a regulated way depending on various contexts. When expressed by mesenchymal cells it can instruct epithelial morphogenesis at epithelial mesenchymal interfaces, and Syntaxin-2 is protein that is essential for epithelial cell and tissue morphogenesis and synaptic function in nervous system communication. Syntaxin-2 interacts with synaptotagmin-6 (SYT6) and synaptotagmin-8 (SYT8) in calcium dependent exocytosis of synaptic vesicles and may mediate calcium regulation of exocytosis in the acrosomal reaction in sperm cells as well. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Syntaxin-2 Antibody, clone 2F2.1 is a highly specific mouse monoclonal antibody, that targets Syntaxin 2 & has been tested in western blotting & ICC. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Syntaxin-2 in SH-SY5Y cells. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human Syntaxin-2. |
| Clone | 2F2.1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~ 33 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in SH-SY5Y cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Syntaxin-2 in 200 µg of SH-SY5Y cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |