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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT39 |
| Description | Anti-Laminin alpha 5 Antibody, clone 4B12 |
| Alternate Names |
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| Background Information | Laminin alpha 5 (α5) is a member of the laminin family of extracellular matrix glycoproteins. Laminin’s are important in cell migration, differentiation and in tumor cell incursion. Research shows that laminin alpha 5 may be an early marker of sexual differentiation. In rat, the laminin alpha 5 chain seems to halt expression in the basement membrane of Sertoli cells when secretion of AMH begins but expression increases two weeks after birth when AMH secretion stops. Laminin alpha 5 shows up early during lung development in mice. It has also been linked to inflammatory cell chemotaxis and metalloproteinase activity and may play a role in creating and sustaining mucosal patterns of the mouse small intestine. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1λ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Laminin alpha 5 Antibody, clone 4B12 is validated for use in IH for the detection of Laminin alpha 5. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected Laminin alpha 5 in human blood vessels and metastatic colorectal adenocarcinoma tissues. |
| Biological Information | |
|---|---|
| Immunogen | Laminin purified from human placenta |
| Clone | 4B12 |
| Host | Mouse |
| Isotype | IgG1λ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Components of the extracellular matrix exert myriad effects on tissues throughout the body. In particular, the laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in such diverse organs as the kidney, lung, skin, and nervous system. It is thought that laminins mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. The protein encoded by this gene belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one of them has not been determined. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. SUBUNIT STRUCTURE: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Alpha-5 is a subunit of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). SUBCELLULAR LOCATION: Secreted › extracellular space › extracellular matrix › basement membrane. Note: Major component. TISSUE SPECIFICTY: Expressed in heart, lung, kidney, skeletal muscle, pancreas, retina and placenta. Little or no expression in brain and liver. DOMAIN: Domain G is globular and is part of the major cell-binding site located in the long arm of the laminin heterotrimer. SEQUENCE SIMILARITIES: Contains 22 laminin EGF-like domains. Contains 5 laminin G-like domains. Contains 1 laminin IV type A domain. Contains 1 laminin N-terminal domain. SEQUENCE CAUTION: The sequence CAC22310.1 differs from that shown. Reason: Erroneous gene model prediction. |
| Molecular Weight | 400 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human precancerous colorectal lesion tissue. Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected Laminin alpha 5 in human precancerous colorectal lesion tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |