Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC, IH(P), RIA, IF, IP | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT329 |
| Description | Anti-Cytokeratin 8 Antibody, clone TROMA-1 |
| Alternate Names |
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| Background Information | Keratin, type II cytoskeletal 8 (UniProt: P11679; also known as Cytokeratin endo A, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8) is encoded by the Krt8 (also known as Krt2-8) gene (Gene ID: 16691) in murine species. Cytokeratins belong to a diverse group of intermediate filaments that participate in differentiation and maintain the structural integrity of epithelial cells. Cytokeratins share a common structural organization: a central a-helical rod domain flanked by head and tail domains. An important unique feature of cytokeratins is their epithelial cell-type-specific expression. CK8 is a heterotetramer of two type I and two type II keratins. It is expressed in abundance in the epithelia of colon, bladder, ileum, and stomach. CK8 together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. CK8 is often used together with keratin 18 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. CK8 expression has been correlated with malignancy in leukoplakia and carcinomas of the head and neck and its expression has been observed in all non-small-cell lung cancers. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This rat monoclonal Anti-Cytokeratin 8, clone TROMA-1, Cat. No. MABT329, is a rat monoclonal antibody that detects cytokeratin-8 in human and murine cells & is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Radioimmunoassay, & WB. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A representative lot detected Cytokeratin 8 in Immunocytochemistry applications (ODriscoll, C.M., et. al. (2013). Biochim Biophys Acta. 1833(3):460-7; Wang, X., et. al. (2004). J Cell Sci. 117(Pt 1):53-62). Western Blotting Analysis: A representative lot detected Cytokeratin 8 in Western Blotting applications (Helenius, T.O., et. al. (2016). Cells. 5(3); ODriscoll, C.M., et. al. (2013). Biochim Biophys Acta. 1833(3):460-7; Tao, G.Z., et. al. (2003). J Cell Sci. 116(Pt 22):4629-38; Maurer, J., et. al. (2008) PLoS One. 3(10):e3451). Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Cytokeratin 8 in HepG2 cells. Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Cytokeratin 8 in mouse lung tissue. Immunoprecipitation Analysis: A representative lot detected Cytokeratin 8 in Immunoprecipitation applications (Brulet, P., et. al. (1982). Proc Natl Acad Sci USA. 79(7):2328-32). Radioimmunoassay Analysis: A representative lot detected Cytokeratin 8 in Radioimmunoassy applications (Brulet, P., et. al. (1982). Proc Natl Acad Sci USA. 79(7):2328-32). Immunofluorescence Analysis: A representative lot detected Cytokeratin 8 in Immunofluorescence applications (Helenius, T.O., et. al. (2016). Cells. 5(3); Tao, G.Z., et. al. (2003). J Cell Sci. 116(Pt 22):4629-38; Lin, Y.M., et. al. (2008). J Cell Sci. 121(Pt 14):2382-93; Maurer, J., et. al. (2008) PLoS One. 3(10):e3451; Nuotio-Antar, A.M., et. al. (2006). J Steroid Biochem Mol Biol. 99(2-3):93-9; Tamai, Y., et. al. (2000). J Cell Biol. 151(3):563-72). Immunohistochemistry Analysis: A representative lot detected Cytokeratin 8 in Immunohistochemistry applications (Szeder, V., et. al. (2003). Dev Biol. 253(2):258-63; Tao, G.Z., et. al. (2003). J Cell Sci. 116(Pt 22):4629-38; Nuotio-Antar, A.M., et. al. (2006). J Steroid Biochem Mol Biol. 99(2-3):93-9; Tamai, Y., et. al. (2000). J Cell Biol. 151(3):563-72). |
| Biological Information | |
|---|---|
| Immunogen | Intermediate filaments extracted from TDM1 Trophoblastoma cells. |
| Clone | TROMA-1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone TROMA-1 detects cytokeratin-8 in human and murine cells. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~54 kDa observed; 54.57 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 0.5 ug/mL of this antibody detected Cytokeratin 8 in 10 µg of HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |