Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ICC, IF, IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT1354 |
| Description | Anti-CP110 Antibody, clone 140-195-5 |
| Alternate Names |
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| Background Information | Centriolar coiled-coil protein of 110 kDa (UniProt: O43303; also known as Centrosomal protein of 110 kDa, CP110, Cep110) is encoded by the CCP110 (also known as CEP110, CP110, KIAA0419) gene (Gene ID: 9738) in human. CP110 is a cytoplasmic protein that is essential for centrosome duplication at different stages of procentriole formation. It acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation. It is also involved in the assembly of the mother centriole subdistal appendages thereby affecting the fusion of recycling endosomes to basal bodies during cilia formation. Two isoforms of CP110 have been reported that are produced by alternative splicing. CP110 is highly expressed in testis and intermediate level of expression is observed in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes. CP110 levels re up-regulated during the transition from G1 to S phase of the cell cycle and highest levels are reported in S phase, following which the levels decline significantly. During the G2 phase it is ubiquitinated that leads to its proteasomal degradation, which prevents centrosome reduplication. And, de-ubiquitinated by USP33 in S and G2/M phase stabilizes CCP110 during the period when centrioles duplicate and elongate. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CP110, clone 140-195-5 , Cat. No. MABT1354, is a mouse monoclonal antibody that detects Centriolar coiled-coil protein of 110 kDa and has been tested for use in immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot detected CP110 in HEK293T cell lysate (Courtesy of Prof. Erich A. Nigg, Biozentrum, University of Basel, Switzerland). Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected CP110 in U2OS cell line. Western Blotting Analysis: A representative lot detected CP110 in Western Blotting applications (Bauer, M., et. al. (2016). EMBO J. 35(19):2152-2166). Immunofluorescence Analysis: A representative lot detected CP110 in U20S cells (Courtesy of Prof. Erich A. Nigg, Biozentrum, University of Basel, Switzerland). Western Blotting Analysis: A representative lot detected CP110 in 293T cell lysate (Courtesy of Prof. Erich A. Nigg, Biozentrum, University of Basel, Switzerland). Immunofluorescence Analysis: A representative lot detected CP110 in Western Blotting applications (Bauer, M., et. al. (2016). EMBO J. 35(19):2152-2166; Arquint, C., et. al. (2014). Curr Biol. 24(4):351-60). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged recombinant fragment corresponding to the first 149 amino acids from the N-terminal region of human Centriolar coiled-coil protein of 110 kDa. |
| Epitope | N-terminus |
| Clone | 140-195-5 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 140-195-5 detects Centriolar coiled-coil protein of 110 kDa (CP110) in human cells., It targets an epitope within the first 149 amino acids from the N-termina region. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~150 kDa observed; 113.42 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cell lysate. Western Blotting Analysis: A 1:250 dilution of this antibody detected CP110 in HEK293 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |