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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IF, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT118 |
| Description | Anti-Desmoglein-1 Antibody, clone 32-2B |
| Alternate Names |
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| Background Information | Desmoglein-1 (DSG1) is a transmembrane glycoprotein belonging to the family of cadherins. It is one of the main structural components of desmosomal junctions which are abundant in tissues undergoing frequent mechanical stress, such as the heart, epidermis, lymphatic endothelial cells, and meninges. The desmoglein-1 subtype is highly expressed in the epidermis. Like other cadherins, desmoglein-1 contains multiple calcium binding sites and a cell-adhesion recognition site which facilitates intercellular adhesions. Desmoglein-1 is regulated by calcium and PKCα signaling, and is also coupled to intermediate filaments of the cytoskeleton. Desmosomes play a critical role in cell and tissue stability, and may contribute to the progression of cancer. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Desmoglein-1, clone 32-2B detects levels of Desmoglein-1 proteins & has been published & validated for use in WB, IF, IHC & ICC. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Desmoglein-1 in human tonsil tissues. Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Desmoglein-1 in human tonsil tissues. Western Blot Analysis: A representative lot from an independent laboratory detected Desmoglein-1 in bovine epidermal desmosomal cores and in human keratinocyte cell lysates. (Vilela, M. J., et al. (1995). J Cell Sci. 108(Pt 4):1743-1750.). Immunofluorescent Analysis: A representative lot from an independent laboratory detected Desmoglein-1 in MDCK cells (Vilela, M. J., et al. (1995). J Cell Sci. 108(Pt 4):1743-1750.). Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Desmoglein-1 in human epidermal tissue (Maruani, A., et al. (2008). Am J Clin Pathol. 130(3):369-374.). |
| Biological Information | |
|---|---|
| Immunogen | Bovine Desmoglein-1 from nasal epidermis |
| Clone | 32-2B |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human. Predicted to react with Bovine based on immunogen design. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein by similarity. Cell junction > desmosome. TISSUE SPECIFICTY: Epidermis, muzzle, tongue and esophagus. DOMAIN: Calcium may be bound by the cadherin-like repeats. SEQUENCE SIMILARITIES: Contains 4 cadherin domains. |
| Molecular Weight | ~150 kDa observed. This protein may be glycosolated. Uniprot describes a molecular weight at ~112 kDa; however, Desmoglein-1 has been observed at ~150 kDa (Vilela, M. J., et al. (1995). J Cell Sci. 108(Pt 4):1743-1750.). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in human tonsil tissue lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected Desmoglein-1 in 10 µg of human tonsil tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |