Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M | IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS827 |
| Replaces | 05-1152 |
| Description | Anti-ERK1/2 Antibody, clone 16A6.1 |
| Alternate Names |
|
| Background Information | Mitogen-activated protein kinase 3 (UniProt: P21708; also known as EC: 2.7.11.24, MAP kinase 3, MAPK3, ERT2, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, MNK1, Microtubule-associated protein 2 kinase, p44-ERK1) is encoded by the mapk3 (also known as Erk1, Prkm3) gene (Gene ID: 50689) in rat. ERK1 (a.k.a. MAPK 3) and ERK2 (a.k.a. MAPK 1 or MAPK 2) are related serine/threonine kinases of the Ras-Raf-MEK-ERK signaling pathway. ERK1/2 are activated by MEK1/2-catalyzed phosphorylation at their conserved Thr-Glu-Tyr (TEY) dual phosphorylation motif, first at the Tyr residue (human/rat ERK1 Tyr204/Tyr203, human/rat ERK2 Tyr187/Tyr183) and then at the Thr residue (human/rat ERK1 Thr202/Thr201, human/rat ERK2 Thr185/Thr181). ERK1/2 are proline-directed kinases that preferentially catalyze the phosphorylation of Pro-Xxx-Ser/Thr-Pro sequence motif in hundreds of cytoplasmic and nuclear substrates, including transcription factors such as Ets, Elk, and c-Fos. Besides this primary structure requirement, many ERK1/2 substrates possess a D-docking site and/or an F-docking site. A variety of scaffold proteins, including KSR1/2, IQGAP1, MP1, and -Arrestin1/2, also participate in the regulation of ERK1/2 MAP kinase cascade. Ras-Raf-MEK-ERK signaling activity is upregulated in about one-third of all human cancers, and targeted inhibition against components of this signaling pathway represents a popular anticancer strategy. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect ERK1/2 using this mouse monoclonal Anti-ERK1/2 Antibody, clone 16A6.1, Cat. No. MABS827. It is used in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:250-1:1,000 dilution from a representative lot detected ERK1/2 in human testis, human cerebellum, rat cerebral cortex, and mouse small intestine tissues. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of rat ERK1. |
| Clone | 16A6.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 16A6.1 detects both ERK1 and ERK2 in human, mouse, and rat. This clone targets a sequence corresponding to 21 amino acids in the C-terminal region. |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~42 kDa observed; 43.08 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in RAW264.7 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected ERK1/2 in 10 µg of RAW264.7 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |