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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, FC, ICC | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS514 |
| Description | Anti-Keap1 Antibody, clone 144 |
| Alternate Names |
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| Background Information | KEAP1 is an important adapter protein important in regulating the transcription factor NFE2L2/NRF2. Nuclear factor erythroid-2 related factor 2 (NRF2) is a redox-sensitive transcription factor that positively regulates the expression of genes encoding antioxidants, xenobiotic detoxification enzymes, and drug efflux pumps, and confers cytoprotection against oxidative stress and xenobiotics in normal cells. KEAP1 negatively regulates NRF2 activity by targeting it to proteasomal degradation. KEAP1 acts as a substrate adapter protein for the E3 ligase ubiquitin protein complex formed by CUL3 and RBX1 proteins which mediate the ubiquitination and proteasome destruction of NRF2. KEAP1 also targets PGAM5 which is a central mediator of programmed necrosis induced by TNF and reactive oxygen species, so again KEAP1 plays an important role ultimately in cell survival and adaptation to its microenvironment. KEAP1 is widely expressed in many tissues, most strongly in muscle tissues and appears to shuttle from the cytoplasm to the nucleus depending upon the cellular context. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Keap1 Antibody, clone 144 is validated for use in Western Blotting and Flow Cytometry and Immunocytochemistry for the detection of Keap1. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: 4.0 µg of this antibody from a representative lot detected Keap1 in 1X10E6 NIH/3T3 cells. Western Blotting Analysis: A representative lot detected Keap1 in extracts from mouse embreyonic fibroblasts (MEFs) (Watai, Y., et al. (2007). Genes to Cells. 12:1163-1178). Western Blotting Analysis: A representative lot detected Keap1 in transfected HeLa cells (Tsuchiya, Y., et al. (2011). Mole. and Cell Biol. 31(22):4500-4512). Immunocytochemistry Analysis: A representative lot detected Keap1 in mouse embreyonic fibroblasts (MEFs) cells (Watai, Y., et al. (2007). Genes to Cells. 12:1163-1178). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to mouse Keap1. |
| Clone | 144 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Isotype | IgG2bκ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~69 kDa observed. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Raji cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Keap1 in 10 µg of Raji cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |