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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS436 |
| Description | Anti-ATE1 Antibody, clone 6F11 |
| Alternate Names |
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| Background Information | ATE1 plays an important role a specialized ubiquitin mediated protein degradation pathway. ATE1 is involved in the post-translational conjugation of arginine to the N-terminal or mid-chain Aspartate or Glutamate amino acids of proteins. The arginylation is required for effective ubiquitin mediated protein degradation. ATE1 is a central player in the Arg/N-end rule pathway of ubiquitin mediated protein degradation. This system disposes of malformed or misdirected proteins and protein fragments. ATE1 has two isoforms, isoform 1 is nuclear localized and isoform 2 is cytoplasmically localized. ATE1 is widely expressed, and recent research demonstrates that ATE1 mediated arginylation is essential for platelet myosin phosphorylation, clot retraction and thrombosis formation. Recent research also shows that the Arg/N-end rule pathway and ATE1 may play a role in helping to prevent neurodegeneration caused by the buildup of protein fragments found in various neurological disease states such as Alzheimers disease, amyotrophic lateral sclerosis, and Parkinsons disease. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-ATE1 Antibody, clone 6F11 is validated for use in Western Blotting and Immunocytochemistry for the detection of ATE1. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 0.5 µg/mL from a representative lot detected ATE1 in 10 µg of mouse liver tissue lysate. Western Blotting Analysis: A representative lot detected ATE1 in ATE1 isoforms and mouse liver extract tissue lysate (Wang, J., et al. (2011). Chemistry & Biology. 18:121-130). Immunocytochemistry Analysis: A representative lot detected ATE1 in immunostaining and cell extract preparation, immortalized mouse embryonic fibroblasts derived from wild-type and Ate1 cells (Wang, J., et al. (2011). Chemistry & Biology. 18:121-130). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to mouse ATE1. |
| Clone | 6F11 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Isotype | IgG2bκ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~60 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HepG2 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected ATE1 in 10 µg of HepG2 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |