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Millipore Anti-Diglycyl Lysine (Clone Gx41) Antibody Detects Level Of Diglycyl Lysine (Clone Gx41) & Has Been Published & Validated For Use In Wb & Ip. - Mill (Additional S&H or Hazmat Fees May Apply)
Anti-Diglycyl Lysine has been shown to specifically detect peptides derived from the ubiquitinated portion of proteins. Diglycyl Lysine, clone GX41 is thought to find diglycyl-lysine moieties that are created and left behind at places of ubiquitination after digestion with trypsin has occurred.
Product Information
Format
Purified
Control
BSA protein and Diglycine modified BSA
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Diglycyl Lysine (Clone GX41) Antibody detects level of Diglycyl Lysine (Clone GX41) & has been published & validated for use in WB & IP.
Key Applications
Western Blotting
Immunoprecipitation
Application Notes
Immunoprecipitation Analysis: 10 µg of Anti-Diglycyl Lysine immunoprecipitated peptides containing Diglycyl Lysine modification from isolated ubiquitinated proteins which were obtained from a human cell line.
Mass Spectrometry Analysis: A previous lot was used by an independent a laboratory in Mass Spec. Image courtesy of Samie Jaffrey, Cornell Medical College.
Biological Information
Immunogen
Linear peptide corresponding to Diglycyl Lysine.
Clone
GX41
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
Antibody recognizes lysine residues modified by diglycine, a residue left at sites of ubiquitination after trypsin digestions.
Isotype
IgG1κ
Species Reactivity
Rat
All
Human
Mouse
Species Reactivity Note
Demonstrated to react with human, mouse and rat. Broad species cross-reactivity expected based on 100% sequence homology.
Antibody Type
Monoclonal Antibody
Gene Symbol
Diglycyl Lysine
Purification Method
Protein G
Molecular Weight
Molecular weight can vary depending on the size of the protein or peptides containing Diglycyl modified lysine residues.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in BSA protein and Diglycine modified BSA.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Diglycyl Lysine on 10 µg of BSA protein and Diglycine modified BSA.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
