Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M | IH(P), ICC, WB, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS2001 |
| Description | Anti-LRP-1 Antibody, clone 11H4 |
| Alternate Names |
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| Background Information | Prolow-density lipoprotein receptor-related protein 1 (UniProt: Q07954; also known as LRP-1, Alpha-2-macroglobulin receptor1, A2MR, Apolipoprotein E receptor, APOER, CD91) is encoded by the LRP1 (also known as A2MR, APR) gene (Gene ID: 4035) in human. LRP-1 is expressed in most tissues, but is abundant in liver, brain, and lung. LRP1 is synthesized with a signal peptide sequence (a.a. 1-19) and is processed in trans-Golgi network by furin to generate a 515 kDa alpha subunit and an 85 kDa beta subunit. The alpha and beta subunits are non-covalently linked during LRP1 transport to the cell membrane. LRP-1 recognizes and mediates the endocytosis of more than 40 different ligands, including apolipoprotein E (ApoE), APP and amyloid beta. It is the primary receptor mediating transport of amyloid beta peptides across the blood-brain barrier into circulation, thereby clearing them from the brain. LRP-1 is required for early embryonic development and is involved in cellular lipid homeostasis and plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin). Excessive copper accumulation in the brain has been linked with reduced LRP-1 mediated clearance of amyloid beta peptides across the blood brain barrier, which may contribute to complications of Alzheimers disease. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-LRP-1, clone 11H4, Cat. No. MABS2001, is a mouse monoclonal antibody that detects Prolow-density lipoprotein receptor-related protein 1 in human, mouse, and rat and has been tested for use in Immunohistochemistry (Paraffin), Immunocytochemistry, Western Blotting and Immunoprecipitation. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected LRP-1 in human liver tissue. Immunoprecipitation Analysis: A representative lot detected LRP-1 in Immunoprecipitation applications (Nieves, E.C., et. al. (2010). J Biol Chem. 285(17):12595-603). Western Blotting Analysis: A representative lot detected LRP-1 in Western Blotting applications (Spoelgen, R., et. al. (2009). Neuroscience. 158(4):1460-8; Lleó, A., et. al. (2005). J Biol Chem. 280(29):27303-9; Campana, W.M., et. al. (2006). J Nuerosci. 26(43):11197-207). Immunocytochemistry Analysis: A representative lot detected LRP-1 in Immunocytochemistry applications (Lleó, A., et. al. (2005). J Biol Chem. 280(29):27303-9). Immunohistochemistry Analysis: A representative lot detected LRP-1 in Immunohistochemistry applications (Campana, W.M., et. al. (2006). J Nuerosci. 26(43):11197-207). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 13 amino acid from the C-terminal region on human LRP-1. |
| Clone | 11H4 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 11H4 detects LRP-1 protein in human, mouse, and rat tissues. It targets an epitope within 13 amino acids from the C-terminal region. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse, Rat. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 504.61 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human brain tissue. Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected LRP-1 in human brain tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |