Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1905 |
| Description | Anti-TMEPAI Antibody, clone 9F10 |
| Alternate Names |
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| Background Information | Protein TMEPAI (UniProt: Q969W9; also known as Prostate transmembrane protein androgen induced 1, Solid tumor-associated 1 protein, Transmembrane prostate androgen-induced protein) is encoded by the PMEPA1 (also known as STAG1, TMEPAI) gene (Gene ID: 56937) in human. TMEPAI is a type Ib transmembrane protein containing two TMEPAI contains 2 WW-binding motifs and a SMAD interaction motif. The WW-binding motifs mediate its interaction with NEDD4 and the SMAD interaction motif is required for interaction with SMAD2 and SMAD3 and for the negative regulation of TGF-beta signaling. TMEPAI antagonizes TGF-beta signaling by interfering with TGF-beta type I receptor-induced R-SMAD phosphorylation. It competes with SMAD anchor for receptor activation for R-SMAD binding, thereby sequestering R-SMADs from TGF-beta receptor I kinase activation. By acting as a negative regulator of TGF-beta signaling, TMEPAI plays a role in cell proliferation, differentiation, apoptosis, motility, extracellular matrix production, and immunosuppression. Higher expression of TMEPAI is observed in prostate and ovaries and the expression can be up-regulated by androgen and TGF-beta (at protein level).TMEPAI is constitutively and highly expressed in many types of cancer and has been implicated in poor prognosis. TMEPAI is also reported to be involved in down-regulation of the androgen receptor (AR), enhancing ubiquitination and proteasome-mediated degradation of AR. (Ref.: Nguyen THV et al. (2014). Cancer Sci. 105: 334 341; Watanabe, Y et al. (2010). Mol. Cell 37(1): 123-134). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This mouse monoclonal Anti-TMEPAI, Antibody, clone 9F10, Cat. No. MABS1905 is used in Immunocytochemistry, Immunoprecipitation, and Western blotting for the detection of TMEPAI. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot detected TMEPAI in Immunoprecipitation applications (Vo Nguyen, T.T., et. al. (2014). Cancer Sci. 105(3):334-41). Western Blotting Analysis: A representative lot detected TMEPAI in lysate from COS-7 cells overexpressing TEMPAI. (Courtesy of Yukihide Watanabe, Ph.D. and Mitsuyasu Kato, Ph.D., University of Tsukuba, Japan). Western Blotting Analysis: 8 ug/mL from a representative lot detected TMEPAI in TGF-beta stimulated HaCaT cell lysates (Courtesy of Yukihide Watanabe, Ph.D. and Mitsuyasu Kato, Ph.D., University of Tsukuba, Japan). Immunocytochemistry Analysis: A representative lot detected TMEPAI in Immunocytochemistry applications (Vo Nguyen, T.T., et. al. (2014). Cancer Sci. 105(3):334-41). Western Blotting Analysis: A representative lot detected TMEPAI in Western Blotting applications (Vo Nguyen, T.T., et. al. (2014). Cancer Sci. 105(3):334-41). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant fragment corresponding to 39 amino acids from the C-terminal region of human TMEPAI. |
| Clone | 9F10 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 9F10 detects TMEPAI in COS-7 cells overexpressing TEMPAI. It targets an epitope of 39 amino acids from the C-terminal region in isoform 1. Also shows reactivity with isoform 2 that has 100% sequence homology in this epitope region. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human, Monkey. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~32 kDa observed; 31.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in COS-7 overexpressing TMEPAI lysate. Western Blotting Analysis: 0.02 µg/mL of this antibody detected TMEPAI in 45 µg of lysate from COS-7 cells overexpressing TMEPAI. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |