Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1822 |
| Description | Anti-SHMT Antibody, clone 4D2.1 |
| Alternate Names |
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| Background Information | Serine hydroxymethyltransferase, mitochondrial (UniProt: P34897; also known as EC: 2.1.2.1, SHMT, Glycine hydroxymethyltransferase, Serine methylase) is encoded by the SHMT2 gene (Gene ID: 6472) in human. SHMT is involved in the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism. Thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA. SHMT catalyzes the reversible conversion of serine and glycine. It uses pyridoxal-5 -phosphate as a cofactor. SHMT is synthesized with a transit peptide (aa 1-29) that is needed for its mitochondrial translocation. In eukaryotes two forms of SHMT have been reported: cytosolic and mitochondrial. In the presence of bound pyridoxal 5-phosphate it is present in a homotetrameric form and in the absence of pyridoxal 5-phosphate it exists as a homodimer. Pyridoxal 5-phosphate binding mediates an important conformation change, which is essential for its tetramerization. Three isoforms of SHMT have been reported that are produced by alternative splicing. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-SHMT, clone 4D2.1, Cat. No. MABS1822, is a mouse monoclonal antibody that detects mitochondrial Serine hydroxymethyltransferase and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50-250 dilution of this antibody from a representative lot detected SHMT in human colon, human liver, and human pancreas tissue sections. Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SHMT in Jurkat cell lysate. |
| Biological Information | |
|---|---|
| Immunogen | GST/His-tagged recombinant fragment corresponding to 107 amino acids from the C-terminal half of human mitochondrial Serine hydroxymethyltransferase. |
| Clone | 4D2.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 4D2.1 detects human mitochondrial Serine hydroxymethyltransferase. It targets an epitope within 107 amino acids from the C-terminal half. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~56 kDa observed; 55.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Raji cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SHMT in Raji cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |