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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1721 |
| Description | Anti-Potassium Channel Kir3.4 Antibody, clone 8F4.1 |
| Alternate Names |
|
| Background Information | G protein-activated inward rectifier potassium channel 4 (UniProt P48544; also known as Cardiac inward rectifier, CIR, Heart KATP channel, Inward rectifier K(+) channel Kir3.4, GIRK-4, IRK-4, KATP-1, Potassium channel, inwardly rectifying subfamily J member 5) is encoded by the KCNJ5 (also known as GIRK4, LQT13) gene (Gene ID 3762) in human. Inward rectifier K+ channels (Kirs) modulate cell membrane potential and are classified as K+ transport channels (Kir1.x, Kir4.x, Kir5.x, Kir7.x), classic Kir (Kir2.x), G-protein-gated Kirs (Kir2.x and Kir3.x), and ATP-sensitive Kirs (Kir6.x). Kirs mediate diverse physiological functions of both excitable and nonexcitable epithelial cells, such as cell adhesion-migration, volume regulation, apoptosis, and proliferation. While a large fraction of Kir3.4 (GIRK4; KCNJ5) subunits is processed to the cell membrane to form homomultimers, Kir3.4 subunits also assemble into heteromeric channels with Kir3.1 (GIRK1; KCNJ3) subunits in atrial myocytes or Kir3.2 (GIRK2; KCNJ6) in the cerebellum. Upon agonist activation of appropriate GPCRs, heteroterameric Kir3.4 channels are opened via interaction with subunits of the pertussis toxin-sensitive class (Gi/o) G-proteins, resulting in membrane hyperpolarization and/or reduced excitability. Whereas homotetrameric Kir3.4 channels can be activated by high intracellular Na+ independent of G-protein. Human Kir3.4 is a 419-amino acid multi-pass membrane (a.a. 87-111, 136-147, 164-185) protein that contains two small extraceullar regions (a.a. 112-135 and 155-163) and a pore-forming domain (a.a. 148-154), having both its N- and C-terminal ends exposed intracellularly (a.a. 1-86 and a.a. 186-419). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect K(+) channel Kir3.4 using this mouse monoclonal Anti-Potassium Channel Kir3.4, clone 8F4.1, Cat. No. MABS1721, validated for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected potassium channel Kir3.4 in human adrenal gland and kidney tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged human potassium channel Kir3.4 C-terminal end fragment. |
| Clone | 8F4.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 8F4.1 detects human potassium channel Kir3.4 by targeting an epitope within the C-terminal cytoplasmic domain. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Specific Activity | C-terminal region. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Protein G purified |
| UniProt Number |
|
| Molecular Weight | ~47 kDa observed. 47.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human adrenal gland tissue lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected potassium channel Kir3.4 in 10 µg of human adrenal gland tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |