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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1713 |
| Description | Anti-Synaptotagmin-like Protein 4 (SYTL4) Antibody, clone 7D2.2 |
| Alternate Names |
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| Background Information | Synaptotagmin-like protein 4 (UniProt Q96C24; also known as Exophilin-2, Granuphilin) is encoded by the SYTL4 (also known as SLP4) gene (Gene ID 94121) in human. SYLT4 belongs to the synaptotagmin-like protein family, which contains an N-terminal RabBD (Rab-binding) domain and two C-terminal C2 domains. The characteristic feature of this family is the N-terminal domain, which is not found in other C-type tandem C2 proteins. SYLT4 is detected close to the plasma membrane and on secretory granules. Interacts with vesicles containing negatively charged phospholipids in a Ca2+-independent manner. It can bind to both the GTP- and GDP-bound forms of Rab27A and inhibits a specific GTP/GDP exchange cycle required for dense-core vesicle exocytosis. It is the first regulatory in the exocytosis pathway that functions by directly connecting Rab and SNARE. It modulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary. It is not only essential for the docking of insulin granules, but also simultaneously imposes a fusion constraint on them through an interaction with syntaxin-1alpha fusion machinery. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect SYTL4 using this mouse monoclonal Anti-Synaptotagmin-like Protein 4 (SYTL4) Antibody, clone 7D2.2, Cat. No. MABS1713, validated for use in Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 0.5 µg/mL from a representative lot detected synaptotagmin-like protein 4 (SYTL4) in 10 µg of human adrenal gland tissue lysate. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human synaptotagmin-like protein 4 (SYTL4) internal fragment. |
| Clone | 7D2.2 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 7D2.2 epitope resides within an internal region mostly missing in human spliced isoform 2. Reactivity toward isoform 2 has not been confirmed. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~80 kDa observed. 70.02 kDa (human isoform 1) calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected synaptotagmin-like protein 4 (SYTL4) in 10 µg of HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |