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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1685 |
| Description | Anti-ARHGEF28 Antibody, clone 14C10.1 |
| Alternate Names |
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| Background Information | Rho guanine nucleotide exchange factor 28 (UniProt P0C6P5; also known as 190 kDa guanine nucleotide exchange factor, p190-RhoGEF, p190RhoGEF, Rho guanine nucleotide exchange factor) is encoded by the Arhgef28 (also known as Rgnef) gene (Gene ID 361882) in rat species. p190RhoGEF is an ubiquitously expressed protein that belongs to the same subclass of Dbl family GEFs as Lbc, Lfc, GEFH-1, and p115RhoGEF. It contains several potential regulatory motifs, including an N-terminal leucine-rich region, a cysteine-rich zinc-finger domain and a C-terminal region that may form an alpha-helical coiled-coil. p190RhoGEF binds to RhoA, but not with Rac and Cdc42. When overexpressed in neuronal cells, it induces cell rounding and inhibits neurite outgrowth. Endogenous p190RhoGEF is reported to localize to distinct RhoA-containing regions at the plasma membrane, to the cytosol and along microtubules. In vitro and in vivo binding experiments show that p190RhoGEF directly interacts with microtubules via its C-terminal region adjacent to the catalytic Dbl homology/pleckstrin homology domain. Ref.: Van Horck FP et al. (2001). J. Biol. Chem. 276, 4948-4956. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect ARHGEF28 using this mouse monoclonal Anti-ARHGEF28, clone 14C10.1, Cat. No. MABS1685, validated for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ARHGEF28 in rat heart and kidney tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant rat ARHGEF28 fragment encompassing the N-terminal portion of microtubles-interacting domain, excluding sequence C-terminal to the RNA-binding region. |
| Clone | 14C10.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 14C10.1 targets an epitope within the N-terminal portion of ARHGEF28 microtubles-interacting domain. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Mouse, Rat. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~190 kDa observed. 190.8 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in PC12 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ARHGEF28 in 10 µg of PC12 rat pheochromocytoma cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |