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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Mk, B, Ht, R, M | WB | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1326 |
| Description | Anti-Importin alpha-3/Qip1 Antibody, clone 3D10 |
| Alternate Names |
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| Background Information | Importin subunit alpha-3 (UniProt O35343; also known as Importin alpha Q1, Karyopherin subunit alpha-4, Qip1) is encoded by the Kpna4 (also known as Qip1) gene (Gene ID 16649) in murine species. The transport of macromolecules between cytoplasm and nucleus is mediated by importins and exportins and regulated by the small GTPase Ran. Importins bind their cargoes with either nuclear localization signal (NLS) or M9 domain-containing hnRNP proteins in the cytoplasm, while exportins target their substrates via their nuclear export signal (NES) or M9 domain inside the nucleus. Qip1/KPNA4 belongs to the importin- /karyopherin- (KPNA) family of adaptor proteins that interact with both monopartite and bipartite types of classical nuclear localization signals (cNLSs). The seven KPNA family members are divided into the 1 (KPNA1/5/6), 2 (KPNA2/7), and 3 (KPNA3/4) subfamilies based on structural and functional similarities. KPNA members mediate substrate cargo nuclear import by bridging substrate cargo and the Ran-karyopherin- complex. Both KPNA3 and Qip1/KPNA4 are reported to target methyl-CpG-binding protein 2 (MeCp2) bipartite NLS (255-RKAEADPQAIPKKRGRK-271). Qip1/KPNA4 can ineract with both wild-type and MeCp2 R270X mutants found in Rett syndrome (RTT) patients, while KPNA3 binds only wild-type, but not MeCp2 R270X mutants. Human and murine Qip1 contains an NLS (a.a. 43-52) in its importin- -binding (IBB) domain (a.a. 2-58), followed by ten ARM domains (a.a. 66-485), one majore and one minor NLS-binding site (a.a. 137-229 and a.a. 306-394). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Importin subunit alpha-3/Qip1 using this rat monoclonal Anti-Importin alpha-3/Qip1, clone 3D10, Cat. No. MABS1326, validated for use in Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected recombinant mouse importin 3/Qip1, but not importin 1/Rch1, importin 5/NPI-1, or importin 7/S2 GST fusion proteins (Sakaguchi, N., et al. (2003). Hybrid. Hybridomics. 22(6):397-400). Western Blotting Analysis: A representative lot detected endogenous importin 3/Qip1 in human HeLa, monkey COS-7, mouse L-929, rat NRK , hamster BHK, and bovine MDBK cell lysates (Sakaguchi, N., et al. (2003). Hybrid. Hybridomics. 22(6):397-400). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant full-length mouse Importin alpha-3/Qip1. |
| Clone | 3D10 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone 3D10 detected recombinant mouse importin 3/Qip1, but not importin 1/Rch1, importin 5/NPI-1, or importin 7/S2 GST fusion proteins (Sakaguchi, N., et al. (2003). Hybrid. Hybridomics. 22(6):397-400). |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse, Rat, Monkey, Bovine, Hamster. Predicted to react with Canine based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~58 kDa observed. 57.76 kDa (human) and 57.79 kDa (mouse & rat) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected in 10 µg of HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |