Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, WB, ICC, IH(P), ELISA | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1300 |
| Description | Anti-asparagine synthetase Antibody, clone Z5808 |
| Alternate Names |
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| Background Information | Asparagine synthetase [glutamine-hydrolyzing] (UniProt: P08243; also known as EC:6.3.5.4, Cell cycle control protein TS11, Glutamine-dependent asparagine synthetase) is encoded by the ASNS (also known as TS11) gene (Gene ID: 440) in human. Asparagine synthetase catalyzes the biosynthesis of L-asparagine from L-aspartate in an ATP-dependent manner. It contains one asparagine synthetase domain and one glutamine amidotransferase type-2 domain. ASNS transcription is enhanced by amino acid deprivation via a signal transduction pathway that senses amino acid availability. Mutations in ASNS gene are reported to result in asparagine synthetase deficiency that is characterized by microcephaly, severely delayed psychomotor development, progressive encephalopathy, and hyperekplexic activity. There has been much interest in the development of asparagine synthetase inhibitors for treatment of asparaginase-resistance leukemias and levels of asparagine synthetase have been considered as a potential biomarker in ovarian cancers. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2a in PBS with 0.01% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This mouse monoclonal Anti-asparagine synthetase, clone Z5808, Cat. No. MABS1300 has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting for the detection of human Asparagine Synthetase. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected asparagine synthetase in human tonsil and human uterus tissues. Immunocytochemistry Analysis: A representative lot detected asparagine synthetase in Immunocytochemistry applications (Kusano-Arai, O., et. al. (2012). Hybridoma (Larchmt). 31(5):325-32). Flow Cytometry Analysis: A representative lot detected asparagine synthetase in Flow Cytometry applications (Kusano-Arai, O., et. al. (2012). Hybridoma (Larchmt). 31(5):325-32). ELISA Analysis: A representative lot detected asparagine synthetase in ELISA applications (Kusano-Arai, O., et. al. (2012). Hybridoma (Larchmt). 31(5):325-32). Western Blotting Analysis: A representative lot detected asparagine synthetase in Western Blotting applications (Kusano-Arai, O., et. al. (2012). Hybridoma (Larchmt). 31(5):325-32). |
| Biological Information | |
|---|---|
| Immunogen | N-asparagine synthetase-budded virus. |
| Clone | Z5808 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone Z5808 detects Asparagine synthetase in human creast cancer, human tonsil, and human uterus. It targets an epitope within 50 amino acids from the N-terminal end. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Ammonium Sulfate |
| UniProt Number |
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| Molecular Weight | 64.37 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human breast cancer tissues. Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected asparagine synthetase in human breast cancer tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |