Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, NhP | ELISA, IP, WB | M | Biotin | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1284-B |
| Description | Anti-Apolipoprotein(a) Antibody, clone LPA4 Antibody, Biotin Conjugate |
| Alternate Names |
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| Background Information | Apolipoprotein(a) (UniProt P08519; also known as Apo(a), Lp(a)) is encoded by the LPA gene (Gene ID 4018) in human. Apo(a) is the main constituent of lipoprotein(a) (Lp(a)). It is synthesized with a signal peptide (aa 1-19) that is subsequently cleaved off. Lipoprotein (a)/Lp(a) is composed of Apo(a) covalently bound to apolipoprotein B-100/ApoB via a single disulfide bond on kringle (K)IV type 9 (KIV9) to a site near ApoB low density lipoprotein (LDL) receptor-binding site. Structurally, Apo (a) contains ten subtypes of KIV sequences, with the KIV2 subtype present in multiple identical repeats, followed by a KV sequence and an inactive protease domain. Elevated levels of Lp(a) are considered as a risk factor in the development of cardiovascular diseases and peripheral arterial disease. Lp(a) is a preferential lipoprotein carrier of oxidized phospholipids (OxPLs) and circulating levels of oxidized LDL are strongly associated with angiographically documented coronary artery disease. Apo(a) is known to be proteolytically cleaved. Apo(a) fragments accumulate in atherosclerotic lesions, where they may promote thrombogenesis. |
| Product Information | |
|---|---|
| Format | Biotin |
| Presentation | Purified mouse monoclonal antibody IgG1 in PBS with 1.5% BSA and 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Apolipoprotein(a), clone LPA4, Biotin Conjugate, Cat. No. MABS1284-B, is a mouse monoclonal antibody that detects Apolipoprotein (a)/Apo(a)/Lp(a) in human and non-human primates and has been tested for use in ELISA, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | ELISA Analysis: A representative lot detected Apolipoprotein(a) in ELISA applications (Pedersen, T.X., et. al. (2010). J Lipid Res. 51(10):2967-75; Leibundgut, G., et. al. (2013). J Lipid Res. 54(10):2815-30). ELISA Analysis: 0.1 µg/mL from a representative lot detected Apolipoprotein(a) in Patient Lp(a) Plasma sample (Courtesy of Dr. Sotirios Tsimikas, M.D., University of California at San Diego, USA). Immunoprecipitation Analysis: A representative lot detected Apolipoprotein(a) in Immunoprecipitation applications (Tsimikas, S., et. al. (2004). Circulation. 109(25):3164-70). Western Blotting Analysis: A representative lot detected Apolipoprotein(a) in Western Blotting applications (Leibundgut, G., et. al. (2013). J Lipid Res. 54(10):2815-30). |
| Biological Information | |
|---|---|
| Immunogen | Human Lipoprotein a (Lp(a)). |
| Clone | LPA4 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone LPA4 detects Apolipoprotein(a) in human and non-human primates. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human, Non-Human Primates. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 501.32 kDa calculated for Apolipoprotein A |
| Product Usage Statements | |
|---|---|
| Quality Assurance | N/A |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |