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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | IH(P), EM, IP, WB, ELISA | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1273 |
| Description | Anti-Thrombomodulin Antibody, clone 411-201B |
| Alternate Names |
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| Background Information | Thrombomodulin (UniProt P15306; also known as CD141, Fetomodulin, SnoRNA MBII-339, TM) is encoded by the Thbd gene (Gene ID 21824) in murine species. Thrombomodulin (TM) is an endothelial cell surface protein that functions to suppress the propagation of thrombin (activated blood-coagulation factor II) generation by acting as a thrombin receptor. Formation of the TM-thrombin complex abolishes thrombins procoagulant activity by altering its substrate specificity, leading to proteolytic cleavage of protein C into the natural anticoagulant termed "activated protein C". Together with protein S, activated protein C proteolytically cleaves activated coagulation factors Va and VIIIa, thereby preventing the generation of activated coagulation factors Xa and thrombin. Murine thrombomodulin is initially produced with an N-terminal propeptide (a.a. 1-16) sequence, the removal of which yields the mature protein with a large extracellular region (a.a. 17-517), a transmembrane segment (a.a. 518-541), and a short cytoplamic tail (a.a. 542-577). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Thrombomodulin Antibody, clone 411-201B is an antibody against Thrombomodulin for use in Immunohistochemistry (Paraffin), Electron Microscopy, Immunoprecipitation, Western Blotting, ELISA. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Thrombomodulin in human kidney tissue. Electron Microscopy: A representative lot detected thrombomodulin immunoreactivity associated with capillary endothelial cells by EM using frozen mouse lung sections (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701). Immunoprecipitation Analysis: A representative lot immunoprecipitated the 112 kDa thrombomodulin from normal mouse lung fibroblast (NMLF) lysates, but not from thrombomodulin-depleted NMLF lysates by performing IP with clone 273-34A (Cat. No. MABS1245) prior to IP with clone 411-201B (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701). Western Blotting Analysis: A representative lot detected thrombomodulin (P112) in the normal mouse lung fibroblast (NMLF) immunoprecipitates obtained with either clone 411-201B or clone 273-34A (Cat. No. MABS1245) (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701). Immunohistochemistry Analysis: Representative lots and clone 273-34A (Cat. No. MABS1245) showed similar staining pattern using both frozen and paraffin-embedded mouse lung sections (Ford, V.A., et al. (1992). J. Biol. Chem. 267(8):5446-5450; Rorvik, M.C., et al. (1988). J. Histochem. Cytochem. 36(7):741-749; Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701). ELISA Analysis: Representative lots were used as the detection antibody for the detection of thrombomodulin in mouse tissue samples by sandwich ELISA using clone 273-34A (Cat. No. MABS1245) as the capture antibody and an HRP-conjugated secondary antibody, or by sandwich radio-immunoassay employing 125I-labeled clone 411-201B without an enzyme-conjugated secondary antibody (Isermann, B., et al. (2001). Development. 128(6):827-838; Conway, E.M., et al. (1999). Blood. 93(10):3442-3450; Weiler-Guettler, H., et al. (1996). Circ. Res. 78(2):180-187; Ford, V.A., et al. (1992). J. Biol. Chem. 267(8):5446-5450). |
| Biological Information | |
|---|---|
| Immunogen | Normal mouse lung homogenate. |
| Epitope | Extracellular domain. |
| Clone | 411-201B |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
|
| Molecular Weight | 61.87 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in mouse lung tissue. Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Thrombomodulin in mouse lung tissue. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |