| Description | |
|---|---|
| Catalogue Number | MABS1268 |
| Description | Anti-MCRS1 Antibody, clone DON193B/C9 |
| Alternate Names |
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| Background Information | Microspherule protein 1 (UniProt: Q96EZ8; also known as 58 kDa microspherule protein, Cell cycle-regulated factor p78, INO80 complex subunit J, MCRS1) is encoded by the MCRS1 (also known as INO80Q, MSP58) gene (Gene ID: 10445) in human. MCRS1 is a nucleolar protein that is detected in testis, and at lower levels in spleen, thymus, prostate, uterus, small intestine, colon and leukocytes. Its highest levels are reported in the early S phase and it becomes undetectable in the G2 phase. Four isoforms of MCRS1 have been reported that are produced by alternative splicing. MCRS1 contains a nucleolar localization motif (aa 113-123), a coiled-coil domain (aa 301-335), and a forkhead associated (FHA) domain (aa 363-419). MCRS1 exhibits transforming activity, which is suppressed by phosphatase and tensin homologue (PTEN). It can modulate the transcription repressor activity of DAXX by recruiting it to the nucleolus. MCRS1 forms a complex with Mi-2 beta, and RET finger protein (RFP) and co-localize in the Nucleolus. This complex activates rRNA promoter activity. (Ref.: Shimono, K., et al. (2005). J. Biol. Chem. 280(47); 39436-39447). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MCRS1, clone DON193B/C9, Cat. No. MABS1268, is a rat monoclonal antibody that detects Microspherule protein 1 and has been tested for use in Western Blotting. |
| Key Applications |
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| Application Notes | Western Blotting Analysis: A representative lot detected MCRS1 in human tonsil, human spleen, HeLa cell, and Ramos cell lysates (Courtesy of Giovanna Roncador, Ph.D., CNIO, Madrid, Spain). |
| Biological Information | |
|---|---|
| Immunogen | A mixture of GST-tagged full length recombinant human Microspherule protein 1 and GST-tagged C- and N-terminal recombinant fragments of human Microspherule protein 1. |
| Epitope | unknown |
| Clone | DON193B/C9 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone DON193B/C9 detects Microspherule protein 1 in human cells. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~52 kDa observed; 51.80 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HCT116 cell lysate. Western Blotting Analysis: 4 µg/mL of this antibody detected MCRS1 in 10 µg of HCT116 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 100 μg |