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Millipore Anti-Cyp2j2, Clone 6-5-20-8, Cat. No. Mabs1242, Is A Mouse Monoclonal Antibody That Targets Cytochrome P450 2j2 And Is Tested For Use In Inhibition Assay And Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Cytochrome P450 2J2 (UniProt: P51589; also known as EC:1.14.14.1, Arachidonic acid epoxygenase, CYPIIJ2) is encoded by the CYP2J2 gene (Gene ID: 1573) in human. Cytochrome P450 2J2 is an endoplasmic reticulum microsomal enzyme that metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. It is responsible for the epoxidation of endogenous cardiac arachidonic acid pools. It is highly expressed in heart and is present at lower levels in liver, ileum, jejunum, colon, and kidney. It is reported to activate the nuclear peroxisome proliferator-activated receptor alpha (PPAR alpha) and hence plays a role in lipid metabolism and inflammation. Overexpression of CYP2J2 in vivo is shown to reverse several pathological processes in animals, including hypertension, other cardiovascular pathologies, and insulin resistance.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Quality Level
MQ100
Applications
Application
Anti-CYP2J2, clone 6-5-20-8, Cat. No. MABS1242, is a mouse monoclonal antibody that targets cytochrome P450 2J2 and is tested for use in Inhibition Assay and Western Blotting.
Key Applications
Western Blotting
Inhibition
Application Notes
Inhibition Analysis: A representative lot detected CYP2J2 in Inhibition applications (Xiao, Y.F., et. al. (2004). Mol Pharmacol. 66(6):1607-16).
Western Blotting Analysis: A representative lot detected CYP2J2 in Western Blotting applications (Xiao, Y.F., et. al. (2004). Mol Pharmacol. 66(6):1607-16).
Biological Information
Immunogen
A full-length recombinant human cytochrome P450 2J2.
Clone
6-5-20-8
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 6-5-20-8 detects cytochrome p450 2J2 in human and murine species.
Isotype
IgG1κ
Species Reactivity
Mouse
Human
Species Reactivity Note
Human, Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_000766
Gene Symbol
CYP2J2
Purification Method
Protein G purified
UniProt Number
P51589
Molecular Weight
~52 kDa observed; 57.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in mouse heart tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected CYP2J2 in 20 µg of mouse heart tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
