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Millipore Anti-Pycr1 Antibody, Clone 2d9.1 Is An Antibody Against Pycr1 For Use In Western Blotting, Immunohistochemistry (Paraffin). - Mill (Additional S&H or Hazmat Fees May Apply)
Pyrroline-5-carboxylate reductase 1, mitochondrial (EC 1.5.1.2; UniProt P32322; also known as P5C reductase 1, P5CR 1) is encoded by the PYCR1 (also known as ARCL2B, ARCL3B) gene (Gene ID 5831) in human. Pyrroline-5-carboxylate (P5C) reductase (PYCR1) is a mitochondrial enzyme that utilizes NAD(P)H as cofactor and catalyzes the convertion of P5C to L-proline, as well as the coversion of Δ1-piperideine-6-carboxylate (P6C) to L-pipecolic acid, both of which are intermediates in the catabolism pathway of the essential amino acid L-lysine. PYCR1 gene mutations are associated with the autosomal recessive disorder cutis laxa type II, menifested with premature aging features such as joint laxity and wrinkled/lax skin, as well as developmental delay.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-PYCR1 Antibody, clone 2D9.1 is an antibody against PYCR1 for use in Western Blotting, Immunohistochemistry (Paraffin).
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to the C-terminal of human PYCR1.
Epitope
C-terminal
Clone
2D9.1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Expected to react with spliced isoforms 1 and 3, but not isoform 2, reported by UniProt P32322).
Isotype
IgG2bκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001269209
Gene Symbol
PYCR1
Purification Method
Protein G Purified
UniProt Number
P32322
Molecular Weight
~33 kDa observed. 33.36 kDa (isoform 1) and 35.98 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates. Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in K562 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PYCR1 in 10 µg of K562 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
