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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1136 |
| Description | Anti-Cathelicidin/FALL-39/LL-37 Antibody, clone 8C10.1 |
| Alternate Names |
|
| Background Information | Cathelicidin antimicrobial peptide (UniProt P49913; also known as 18 kDa cationic antimicrobial protein, CAP-18, hCAP-18) is encoded by the CAMP (also known as CAP18, FALL39, LL37) gene (ORF HSD26; Gene ID 820) in human. The human cathelicidin antimicrobial protein 18 (hCAP-18) is initially translated as a precursor molecule with a signal peptide (a.a. 1-30) and a propeptide (a.a. 31-131) sequence, the removal of which yields the mature hCAP-18 (a.a. a.a. 132-173). During the inflammatory response, hCAP-18 is proteolytically cleaved into the FALL-39 (a.a 132-170), which is further processed to yield the antimicrobial protein LL-37 (CAP-37; a.a. 134-170). Neutrophils store preformed hCAP-18 within specific granules and release it into inflamed tissue as a result of granule exocytosis. Once released, hCAP-18 is activated by proteinase-3-mediated cleavage, a component of neutrophil primary granules. In addition to its microbicidal and LPS-sequestering activity, LL-37 is also involved in the regulation of innate and humoral immune responses, wound healing, and tissue repair. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Cathelicidin Antibody, clone 8C10.1 is an antibody against Cathelicidin/FALL-39/LL-37 for use in Immunohistochemistry. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Cathelicidin/FALL-39/LL-37 immunoreactivity in human spleen tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the C-terminal sequence of human Cathelicidin/FALL-39/LL-37. |
| Epitope | C-terminal. |
| Clone | 8C10.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 8C10.1 targets a C-terminal epitope present in the prepro- (a.a. 1-173) and pro- (a.a. 31-173) forms of hCAP-18, as well as the processed antibacterial peptides FALL-39 (a.a 132-170) and LL-37 (a.a. 134-170), but not the preprodomain (a.a. 1-131) or prodomain (a.a. 31-131) fragment. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| Molecular Weight | 19.30 kDa (prepro-hCAP-18), 16.02 kDa (pro-hCAP-18), 4.712 kDa (FALL-39; a.a. 132-170) and 4.493 kDa (LL-37; a.a. 134-170) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human bone marrow tissue. Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Cathelicidin/FALL-39/LL-37 immunoreactivity in human bone marrow tissue sections. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |