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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IHC | M | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN839 |
| Description | Anti-TrpV6 Antibody, clone 4A5.1 |
| Alternate Names |
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| Background Information | Transient receptor potential cation channel subfamily V member 6 (UniProt Q9H1D0; also known as Alu-binding protein with zinc finger domain, Calcium transport protein 1, Calcium transporter-like protein, CaT-L, CaT-like, CaT1, ECaC2, Epithelial apical membrane calcium transporter/channel CaT1, Epithelial calcium channel 2, TrpV6) is encoded by the TRPV6 (also known as ABP/ZF, CAT1, CATL, ECAC2, ZFAB) gene (Gene ID 55503) in human. TRPV6 belongs to the transient receptor potential (TRP) family of membrane proteins. The human TRPV6 is an 765-amino acid, six-transmembrane protein with both N- and C-termini exposed intracellularly. TRPV6 contains a pore-forming loop domain located between the fifth and sixth transmembrane region. Among all the TRPV family members, TRPV5 and TRPV6 are the two highly Ca2+-selective channels that play pivotal roles in the Ca2+-reabsorption in the kidney and intestine, respectively. TRPV6 is found in the apical brush-border membrane of the intestinal enterocytes where it regulates calcium entry into the cells. TRPV6 expression is also reported in human placenta, pancreas, and prostate gland. Vitamin D treatment of human colon cancer Caco-2 cells and duodenal explants results in upregulated TRPV6 mRNA levels. |
| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Presentation | In culture supernatant without preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:500 dilution from a representative lot detected TrpV6 in 10 µg of LNCaP prostate adenocarcinoma and K562 erythroleukemia cell lysates. Immunohistochemistry Analysis: A 1:50-1:250 dilution from a representative lot detected TrpV6 in human pancreas, human small intestine, rat colon tissue, and mouse brain tissues. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human TrpV6 C-terminal fragment. |
| Epitope | C-terminal region. |
| Clone | 4A5.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Expected to react with both spliced isoforms of human TrpV6 reported by UniProt (Q9H1D0). |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~70 kDa observed. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Y79 retinoblastoma cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected TrpV6 in 10 µg of Y79 retinoblastoma cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |