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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | DB, ELISA, IF, IHC, Neutralization Assay, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN819 |
| Description | Anti-Tau Antibody, oligomeric Antibody, clone TOMA-1 |
| Alternate Names |
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| Background Information | Microtubule-associated protein tau (UniProt P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as TAU, MAPT1, MTBTL) gene (Gene ID 4137) in human. In Alzheimers disease (AD) pathology, accumulation of the microtubule-associated protein tau takes place primarily in the neurons. Tau accumulates in both the somatodendritic and axonal domains of neurons. Tau also accumulates in the soma as neurofibrillary tangles (NFTs). Cell death and synaptic lesions occur independently of NFT formation, and research indicates that NFT formation alone is insufficient for neurodegeneration, suggesting that soluble tau aggregates may be the more toxic and pathologically significant tau species. Tau oligomers are neurotoxic when applied extracellularly to cultured neuronal cells, and tau oligomers (but not fibrils) induce neurodegeneration and synaptic and mitochondrial dysfunction in vivo. Moreove, researchers are able to use tau oligomers as a reliable biomarker to differentiate AD brains from age-matched non-AD brains. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse IgG2aκ in buffer containing PBS without preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Tau, oligomeric, clone TOMA-1, Cat. No. MABN819, is amouse monoclonal antibody that targets tau oligomers and has been tested in Dot Blot, ELISA, Immunofluorescence, Immunohistochemistry, Neutralization, and Western Blotting applications. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 20 µg/mL from a representative lot detected upregulated oligomeric tau in soluble PBS brain extract from Alzheimers diseased (AD) human brain (Courtesy of Sengupta, U., et al., Kayed lab, University of Texas, Galveston). Please refer to the following publications regarding the use of TOMA clones in Dot Blot, ELISA, Immunofluorescence, Immunohistochemistry, Neutralization, and Western Blotting applications: 1. Vuono, R., et al. (2015). Brain. 138(Pt 7):1907-1918. 2. Castillo-Carranza, D.L., et al. (2015). J. Neurosci. 35(12):4857-4868. 3. Castillo-Carranza, D.L., et al. (2014). J. Neurosci. 34(12):4260-4272. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant human Tau-441 (Tau-F, Tau-4, 2N4R isoform) oligomers (Lasagna-Reeves, C. A., et al. (2012). FASEB J. 26(5):1946-1959). |
| Clone | TOMA-1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone TOMA-1 (a.k.a. clone H12C10) is among more than 13 hybridomas derived from mice immunized with in vitro generated recombinant Tau-441 (Tau-F, Tau-4, 2N4R isoform) oligomers. These TOMAs are reported to specifically detect oligomeric tau without any significant reactivity toward monomeric tau, tau fibrils, Aβ oligomers, Aβ fibrils, or α-synuclein oligomers (Castillo-Carranza, D.L., et al. (2014). J. Neurosci. 34(12):4260-4272). |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. Predicted to react with Mammals based on high homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein A purified. |
| UniProt Number |
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| Molecular Weight | Variable depending on the size(s) of the oligomer(s). Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Alzheimers diseased human brain lysate. Western Blotting Analysis: 4 µg/mL of this antibody detected Tau oligmers in 25 µg of Alzheimers diseased human brain lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |