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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN807 |
| Description | Anti-EYS Antibody, clone 3G10.1 |
| Alternate Names |
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| Background Information | Protein eyes shut homolog (UniProt Q5T1H1; also known as EGF-like protein 10, EGF-like protein 11, Epidermal growth factor-like protein 10, Epidermal growth factor-like protein 11, Protein spacemaker homolog) is encoded by the EYS (also known as C6orf178, C6orf179, C6orf180, EGFL10, EGFL11, SPAM) gene (ORF UNQ9424/PRO34591, Gene ID 346007) in human. Drosophila eyes shut protein is expressed in photoreceptor cells, the central nervous system, and peripheral sensory ganglia, and is essential for the formation of a matrix-filled intra-rhabdomeral space within the flys retina ommatidia, the loss of spacemaker (spam) converts an open rhabdom system to a closed one. Human EYS/SPAM gene likewise is found to be expressed in the eye and the spinal cord, and mutations in human EYS result in retinitis pigmentosa. Human EYS protein contains 27 EGF-like repeats and five laminin G-like (LG1-5) domains (between EGF repeats 21 & 21, 21 & 22, 23 & 24, 25 & 26, and after repeat EGF repeat 27). The presence of the EGF-like repeats is indicative of a role in the Notch signaling pathway, while EYS LG1-5 bear resemblance to the three agrin laminin G domains that are known to interact with several proteins, including α-dystroglycan at the muscle surface. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-EYS Antibody, clone 3G10.1 is an antibody against EYS for use in Western Blotting, Immunohistochemistry (Paraffin). |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected EYS in human retina tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human EYS fragment corresponding to the EGF-like domains 9-11. |
| Epitope | EGF-like domains 9-11. |
| Clone | 3G10.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Expected to react with spliced isoforms 1 and 3, but not isoforms 2 and 4, reported by UniProt (Q5T1H1). |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | 348.4 kDa (isoform 1) and 350.8 kDa (isoform 3) kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting of EYS recombinant protein. Western Blotting Analysis: 1.0 µg/mL of this antibody detected EYS recombinant protein. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |