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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN787 |
| Description | Anti-Dual oxidase 2, clone Duox S-12 Antibody |
| Alternate Names |
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| Background Information | Dual oxidase 2 is an important enzyme in thyroid hormone biosynthesis and peroxide metabolism. Dual oxidase 2 generates the hydrogen peroxide needed for thyroid peroxidase and lactoperoxidase enzymes as well as processes H2O2 made during metabolic processes. Dual oxidase also plays a role in anti-microbial defense in our mucous membranes by providing the peroxidase for lactoperoxidase’s antimicrobial activities. Dual oxidase 2 is also needed for various extracellular matrix stabilizations and protein cross-linking needs such as during cuticle formation. Dual oxidase 2 is widely expressed in the developing fetus, and in the adult, it is largely expressed in the mucosal and epithelial cells and linings of the gut, lung, salivary glands and testis. Dual oxidase also plays a role in many hypersensitivity and inflammatory diseases and is upregulated in diseases such as colitis and certain cancers. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Dual oxidase 2, clone Duox S-12 Antibody is validated for use in Western Blotting and Immunohistochemistry and Immunocytochemistry for the detection of Dual oxidase 2. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Dual oxidase 2 in human prostate cancer and human breast cancer tissue. Western Blotting Analysis: A representative lot detected Dual oxidase 2 in BxPC-3 cell lysate (Wu, Y., et al. (2013). International Journal of Oncology. 42:1229-1238). Immunocytochemistry Analysis: A representative lot detected Dual oxidase 2 in MIA PACA-2 cell (Wu, Y., et al. (2013). International Journal of Oncology. 42:1229-1238). Immunohistochemistry Analysis: A representative lot detected Dual oxidase 2 MIA PACA-2 tissue (Wu, Y., et al. (2013). International Journal of Oncology. 42:1229-1238). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to the N-terminus of human Dual oxidase 2. |
| Epitope | N-terminus |
| Clone | Duox S-12 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~175 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in BxPC3 treated with IL-4 cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Dual oxidase 2 in 10 µg of BxPC3 treated with IL-4 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |