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Millipore Anti-Wif1 Antibody, Clone 1g5 Is A Highly Specific Mouse Monoclonal Antibody, That Targets Wnt Inhibitory Factor 1 & Has Been Tested In Western Blotting, Ihc & Immunofluorescence. - Mill (Additional S&H or Hazmat Fees May Apply)
WIF1, also known as Wnt inhibitory factor 1 or WIF-1, is a secreted protein that binds to Wnt proteins and inhibits their activity. WNT proteins have been shown to act as an extracellular signaling molecules and may be involved in the control of embryonic development. WIf1 contains an N-terminal WIF domain and 5 epidermal growth factor (EGF)-like domains. It has been implied in recent sutyes that WIF1 expression is downregulated in many types of cancers.
Product Information
Format
Ascites
Control
HeLa, NIH/3T3 and NTERA-2 cell lysates
Presentation
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-WIF1 Antibody, clone 1G5 is a highly specific mouse monoclonal antibody, that targets Wnt inhibitory factor 1 & has been tested in western blotting, IHC & Immunofluorescence.
Key Applications
Western Blotting
Immunohistochemistry
Immunofluorescence
Application Notes
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected WIF1 in HeLa cells.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected WIF1 in human ovarian tumor and lung cancer tissues.
Optimal working dilutions must be determined by end user.
Biological Information
Immunogen
Purified recombinant fragment of human WIF1 expressed in E. Coli.
Clone
1G5
Host
Mouse
Isotype
IgG1
Species Reactivity
Human
Mouse
Antibody Type
Monoclonal Antibody
Gene Symbol
WIF-1
WIF
Purification Method
Unpurified
UniProt Number
Q9Y5W5
Molecular Weight
~43 kDa observed. Uncharacterized bands may appear in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa, NIH/3T3 and NTERA-2 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected WIF1 in HeLa, NIH/3T3 and NTERA-2 cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
