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Millipore Anti-Trka Antibody, Clone 6b2 Is A Highly Specific Mouse Monoclonal Antibody, That Targets Trka & Has Been Tested In Western Blotting, Ihc & Icc. - Mill (Additional S&H or Hazmat Fees May Apply)
TrkA, apparent molecular weight 140 kDa, is a high affinity nerve growth factor (NGF). The Trk proto-oncogene family contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkA binds to nerve growth factor (NGF) and autophosphorylates on tyrosine residues (Tyr490, Tyr674, Tyr675, Tyr751 and Tyr785) to activate multiple downstream effector proteins. Phosphorylation at Tyr490 is required for Shc association and subsequent activation of the Ras-MAP kinase-signaling cascade, which leads to activation of Elk-1-dependent gene transcription and neurite growth. Phosphorylations at Tyr674 and Tyr675 lie within the catalytic domain of TrkA tyrosine kinase and reflect Trk kinase activity. Additionally, phosphorylation at Tyr751 is required for PI3-kinase association and activation of the Akt signaling cascade.
Product Information
Format
Ascites
Control
Human brain tissue lysate
Presentation
Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-TrkA Antibody, clone 6B2 is a highly specific mouse monoclonal antibody, that targets TrkA & has been tested in western blotting, IHC & ICC.
Key Applications
Western Blotting
Immunohistochemistry
Immunocytochemistry
Application Notes
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected TrkA in human brain and rat cerebellum tissues.
Immunocytochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected TrkA in PC-12 cells.
Optimal working dilutions must be determined by end user.
Biological Information
Immunogen
Purified recombinant extracellular fragment of TrkA expressed in HEK293 cells.
Clone
6B2
Host
Mouse
Isotype
IgG1
Species Reactivity
Human
Rat
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_002520
Gene Symbol
NTRK1
MTC
TRK
TRKA
Purification Method
Unpurified
UniProt Number
P04629
Molecular Weight
~87 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 4 isoforms ranging between ~77 kDa and ~87 kDa.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected TrkA in 10 µg of human brain tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
