The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Millipore Anti-Lcn1 Antibody, Clone 10b10 Is A Highly Specific Mouse Monoclonal Antibody, That Targets Lipocalin & Has Been Tested In Western Blotting, Ihc & Immunofluorescence. - Mill (Additional S&H or Hazmat Fees May Apply)
Lipocalin-1 or alternatively known as Tear lipocalin (Tlc), Tear prealbumin (TP) or Von Ebner gland protein (VEG) is encoded by the human gene named LCN1/VEGP and expressed in the salivary and lachrymal glands as well as prostate tissue, but recent research has also shown LCN1 to be produced by other glandular tissues such as the pituitary gland. Lipocalin-1 is part of a larger lipocalin protein family of extracellular proteins and shares the ability to bind hydrophobic ligands and lipids but LCN1 also has other abilities such as being an inhibitor of cysteine proteinases and thus plays a role in the control of inflammation. LCN1 also seems to be important in taste utilization and plays a role in the non-immunological defense against micro-organisms and viruses in the eye and mouth. EMD-Millipore’s Anti-LCN1 mouse monoclonal antibody has been tested in western blot against HEK293 transfected LCN1 lysates and in paraffin embedded immunohistochemistry on human prostate tissue sections and in fluorescent immunocytochemistry on transfected HEK293 cells in culture.
Product Information
Format
Ascites
Control
HEK293 and LCN1-hIgGFc transfected HEK293 cell lysates
Presentation
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-LCN1 Antibody, clone 10B10 is a highly specific mouse monoclonal antibody, that targets Lipocalin & has been tested in western blotting, IHC & Immunofluorescence.
Key Applications
Western Blotting
Immunohistochemistry
Immunofluorescence
Application Notes
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected LCN1 in methanol-fixed HEK293 cells transfected with LCN1-hIgGFc.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected LCN1 in human prostate tissue.
Optimal working dilutions must be determined by end user.
Biological Information
Immunogen
Purified recombinant fragment of human LCN1 expressed in E. Coli.
Clone
10B10
Host
Mouse
Isotype
IgG1
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Gene Symbol
LCN1
VEGP
Purification Method
Unpurified
UniProt Number
P31025
Molecular Weight
~52 kDa observed. Uncharacterized bands may appear in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HEK293 and LCN1-hIgGFc transfected HEK293 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected LCN1-hIgGFc in LCN1-hIgGFc transfected HEK293 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
